CHARACTERISTICS OF 3 HUMAN GASTRIC-CANCER CELL-LINES, NU-GC-2, NU-GC-3 AND NU-GC-4
CHARACTERISTICS OF 3 HUMAN GASTRIC-CANCER CELL-LINES, NU-GC-2, NU-GC-3 AND NU-GC-4
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DOI：
10.1007/bf02471470
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发表时间：
1988-07-01
期刊：
影响因子：
--
通讯作者：
TAKAGI, H
中科院分区：
文献类型：
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作者：
AKIYAMA, S;AMO, H;TAKAGI, H
三种人胃癌细胞系，NU-GC-2。从3例患者手术中获得的癌组织中体外构建NU-GC-3和NU-GC-4。这些病例的胃肿瘤病理表现为低分化腺癌（NU-GC-4为部分印戒细胞癌）。NU-GC-2和NU-GC-4最初是从转移的腹旁淋巴结中获得的，NU-GC03是从臂肌转移瘤中获得的。NU-GC-2和NU-GC-3的细胞呈多角形，呈单层生长。NU-GC-4细胞以球形为主，有少量自由浮动细胞。电镜显示3种细胞系均有上皮特征。NU-GC-2的平均倍增时间为36.1小时，NU-GC- 3的平均倍增时间为38.2小时，NU-GC-4的平均倍增时间为29.9小时。NU-GC-2的模态染色体数为62，NU-GC-3的模态染色体数为58，NU-GC-4的模态染色体数分别为52 ~ 54和53。在同一肿瘤中建立了NU-GC-4体外和体内细胞系。这两种细胞系在形态上很相似，但在核型上略有不同。体外对抗癌药物的敏感性以NU-GC-4最高，NU-GC-2最低。抗肿瘤药物中，丝裂霉素C和阿霉素对3种细胞系的细胞均最有效。
Three human gastric cancer cell lines, NU-GC-2. NU-GC-3 and NU-GC-4 were established in vitro from the cancer tissues obtained from 3 patients during surgery. The pathological findings of the gastric tumors of these cases revealed poorly differentiated adenocarcinoma (and partial signet-ring cell carcinoma of the case of NU-GC-4). NU-GC-2 and NU-GC-4 were originally obtained from metastatic paragastric lymph nodes and NU-GC03 was obtained from a metastatic tumor in the brachial muscle. The cells of NU-GC-2 and NU-GC-3 are polygonal in shape and grow as a monolayer sheet. NU-GC-4 cells, however, are mainly spherical in shape with a few free floating cells. Electron microscopy revealed epithelial characteristics in all 3 cell lines. The average doubling time of NU-GC-2 was 36.1 hours, that of NU-GC--3 was 38.2 hours and that of NU-GC-4 was 29.9 hours. The modal chromosome number of NU-GC-2 was 62, that of NU-GC-3 was 58 and those of NU-GC-4 grown in in vitro and in vivo were 52-54 and 53, respectively. In vitro and in vivo lines of NU-GC-4 were established from the same tumor. These two cell lines are quite similar in morphology, but slightly different in karyotype. The in vitro sensitivity to anticancer agents was highest in NU-GC-4 and lowest in NU-GC-2. Of the anticancer agents, mitomycin C and adriamycin were most effective on the cells of all 3 cell lines.