Nanopores and nucleic acids: prospects for ultrarapid sequencing
Nanopores and nucleic acids: prospects for ultrarapid sequencing
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DOI：
10.1016/s0167-7799(00)01426-8
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发表时间：
2000-04-01
影响因子：
17.3
通讯作者：
Akeson, M
中科院分区：
文献类型：
--
作者：
Deamer, DW;Akeson, M
当DNA和RNA分子被施加的电场以每个分子几百微秒到几毫秒的速率驱动通过纳米孔时，可以检测到它们。纳米孔可以快速区分沿着单链核酸分子的嘧啶和嘌呤区段沿着。单分子的纳米孔检测和表征代表了直接阅读线性聚合物中编码的信息的新方法。如果单核苷酸的分辨率可以实现，就有可能以超过每秒一千个碱基的速率确定核酸序列。
DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers. If single-nucleotide resolution can be achieved, it is possible that nucleic acid sequences can be determined at rates exceeding a thousand bases per second.