Indole-3-Acetic Acid Production by Endophytic Streptomyces sp En-1 Isolated from Medicinal Plants
Indole-3-Acetic Acid Production by Endophytic Streptomyces sp En-1 Isolated from Medicinal Plants
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DOI：
10.1007/s00284-013-0348-z
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发表时间：
2013-08-01
影响因子：
2.6
通讯作者：
Xu, Xudong
中科院分区：
文献类型：
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作者：
Lin, Lan;Xu, Xudong
植物相关放线菌富含吲哚衍生分子，如植物激素吲哚-3-乙酸（IAA）。鉴于目前关于内生放线菌合成IAA的研究较少，本研究对药用植物红豆杉（Taxus chinensis）和黄花蒿（Artemisia annua）内生链霉菌分离株生产IAA的潜力进行了评价。通过高效液相色谱法分析IAA，结合链霉菌通过吲哚-3-乙酰胺（IAM）合成IAA的遗传决定因素IAM的分子筛选方法，我们的数据显示了IAM介导的IAA生物合成在红豆杉（Taxus chinensis）内生链霉菌（Streptomyces sp. En-1）中可能的作用。利用拟南芥与链霉菌的共培养体系，发现En-1在拟南芥的细胞间定植，并分析了内生En-1接种对模式植物的影响。接种En-1对植物的刺激作用表明，产iaa的内生源链霉菌En-1在促进植物生长方面具有重要的经济和农业价值。
Plant-associated actinobacteria are rich sources of bioactive compounds including indole-derived molecules such as phytohormone indole-3-acetic acid (IAA). In view of few investigations concerning the biosynthesis of IAA by endophytic actinobacteria, this study evaluated the potential of IAA production in endophytic streptomycete isolates sourced from medicinal plant species Taxus chinensis and Artemisia annua. By HPLC analysis of IAA combined with molecular screening approach of iaaM, a genetic determinant of streptomycete IAA synthesis via indole-3-acetamide (IAM), our data showed the putative operation of IAM-mediated IAA biosynthesis in Streptomyces sp. En-1 endophytic to Taxus chinensis. Furthermore, using the co-cultivation system of model plant Arabidopsis thaliana and streptomycete, En-1 was found to be colonized intercellularly in the tissues of Arabidopsis, an alternative host, and the effects of endophytic En-1 inoculation on the model plant were also assayed. The phytostimulatory effects of En-1 inoculation suggest that IAA-producing Streptomyces sp. En-1 of endophytic origin could be a promising candidate for utilization in growth improvement of plants of economic and agricultural value.