Recognition of pyrrolysine tRNA by the Desulfitobacterium hafniense pyrrolysyl-tRNA synthetase.
Recognition of pyrrolysine tRNA by the Desulfitobacterium hafniense pyrrolysyl-tRNA synthetase.
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hafniense pyrrolysyl-tRNA合成酶识别吡咯氨酰胺tRNA。
DOI：
10.1093/nar/gkl1151
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发表时间：
2007
影响因子：
14.9
通讯作者：
Soll, Dieter
中科院分区：
文献类型：
--
作者：
Herring, Stephanie;Ambrogelly, Alexandre;Polycarpo, Carla R.;Soll, Dieter
吡咯氨酸酯（PYL）（pyl）是22nd co-Translation插入的氨基酸，以响应UAG Amber终止密码子，将吡咯氨酰基-TRNA合成酶（Pylrs）固定在其认知TRNA上塔）和塔（PYLS）（塔）在所有古细菌的甲麦酸菌科中都发现，并在Deulfitobacterium hafniense中发现。 D. hafniense Pylrs的性质和Trnapyl同一性元素的性质是通过测量用吡咯氨氨酸类似物N-ε-环氧氧化氧氧甲苯和二酰胺的24种突变型Trnapyl物种的能力来确定的。发现受体茎中的对（G1·C72）是主要的身份元素。未观察到T-loop，D-STEM和受体茎。与大多数TRNA不同，规范的T-到D-loop接触不存在。
Pyrrolysine (Pyl), the 22nd co-translationally inserted amino acid, is incorporated in response to a UAG amber stop codon. Pyrrolysyl-tRNA synthetase (PylRS) attaches Pyl to its cognate tRNA, the special amber suppressor tRNAPyl. The genes for tRNAPyl (pylT) and PylRS (pylS) are found in all members of the archaeal family Methanosarcinaceae, and in Desulfitobacterium hafniense. The activation and aminoacylation properties of D. hafniense PylRS and the nature of the tRNAPyl identity elements were determined by measuring the ability of 24 mutant tRNAPyl species to be aminoacylated with the pyrrolysine analog N-ε-cyclopentyloxycarbonyl-l-lysine. The discriminator base G73 and the first base pair (G1·C72) in the acceptor stem were found to be major identity elements. Footprinting analysis showed that PylRS binds tRNAPyl predominantly along the phosphate backbone of the T-loop, the D-stem and the acceptor stem. Significant contacts with the anticodon arm were not observed. The tRNAPyl structure contains the highly conserved T-loop contact U54·A58 and position 57 is conserved as a purine, but the canonical T- to D-loop contact between positions 18 and 56 was not present. Unlike most tRNAs, the tRNAPyl anticodon was shown not to be important for recognition by bacterial PylRS.
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影响因子：
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