REGULATION OF THE HIGHER-ORDER STRUCTURE OF CHROMATIN BY HISTONES-H1 AND HISTONES-H5
REGULATION OF THE HIGHER-ORDER STRUCTURE OF CHROMATIN BY HISTONES-H1 AND HISTONES-H5
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DOI：
10.1083/jcb.90.2.279
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发表时间：
1981-01-01
影响因子：
7.8
通讯作者：
GOULD, H
中科院分区：
文献类型：
--
作者：
ALLAN, J;COWLING, GJ;GOULD, H
利用先前开发的重组技术，制备了含有单一连接物组蛋白H1或H5的鸡红细胞染色质。根据化学和结构标准判断，重组的复合体具有天然鸡红细胞染色质的构象；当两个连接物组蛋白与核小体结合时达到饱和，就像天然红细胞染色质一样，所产生的物质在EM中的外观和相对于游离DNA的线性凝聚因子在定量上相似。在与组蛋白H1或H5相关的连接区，微球菌核酸酶敏感位点的周期为10.4bp，这表明染色质高阶结构中连接区的空间组织类似于分离的核小体。易感部位以不同的频率切割，就像以前发现的核小体核心一样，导致消化中强度的特征分布。连接物DNA中位点的断裂频率还取决于连接物组蛋白的同一性，这表明高阶结构受到相关组蛋白的二次调节。
Chicken erythrocyte chromatins containing a single species of linker histone, H1 or H5, were prepared, using reassembly techniques developed previously. The reconstituted complexes possess the conformation of native chicken erythrocyte chromatin, as judged by chemical and structural criteria; saturation is reached when 2 molecules of linker histone are bound/nucleosome, as in native erythrocyte chromatin, which the resulting material resembles in its appearance in the EM and quantitatively in its linear condensation factor relative to free DNA. The periodicity of micrococcal nuclease-sensitive sites in the linker regions associated with histone H1 or H5 is 10.4 bp, suggesting that the spatial organization of the linker region in the higher-order structure of chromatin is similar to that in isolated nucleosomes. The susceptible sites are cut at differing frequencies, as previously found for the nucleosome cores, leading to a characteristic distribution of intensities in the digests. The scission frequency of sites in the linker DNA depends additionally on the identity of the linker histone, suggesting that the higher-order structure is subject to secondary modulation by the associated histones.