Augmented production of extracellular superoxide by blood isolates of Enterococcus faecalis
Augmented production of extracellular superoxide by blood isolates of Enterococcus faecalis
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DOI：
10.1093/infdis/173.3.743
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发表时间：
1996-03-01
影响因子：
6.4
通讯作者：
Wack, MF
中科院分区：
文献类型：
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作者：
Huycke, MM;Joyce, W;Wack, MF
为了评估肠球菌产生细胞外超氧化物（O-2(-)）的频率，研究人员对多个肠球菌进行了全生物测定，以确定它们以超氧化物歧化酶抑制的方式减少铁细胞色素c的能力。在粪便和临床肠球菌分离株和12种型菌株中，只有粪肠球菌（87/91株和ATCC 19443株）、屎肠球菌（5/13株）、casseliflavus肠球菌（ATCC 25788）和鸡肠球菌（ATCC 35038）产生细胞外O-2（-）。在肠道革兰氏阴性杆菌和革兰氏阳性球菌13种106株中，只有乳酸乳球菌亚种乳酸乳球菌产生胞外O-2（-）。与菌血症和心内膜炎相关的分离株和粪便分离株体外细胞外O-2（-）产生的平均（+/- se）率分别为2.4+/-0.2、1.9+/-0.2和1.5+/-0.3 nmol O-2(-) min(-1) 10(9) cfu(-1) （P = 0.025，方差分析），表明侵袭性与细胞外O-2（-）产生有关。
To assess the frequency of extracellular superoxide (O-2(-)) production by enterococci, multiple species were surveyed in a whole organism assay for their ability to reduce ferricytochrome c in a superoxide dismutase-inhibitable fashion. For stool and clinical enterococcal isolates and 12 type strains, only Enterococcus faecalis (87/91 isolates and ATCC 19443), Enterococcus faecium (5/13 isolates), Enterococcus casseliflavus (ATCC 25788), and Enterococcus gallinarum (ATCC 35038) produced extracellular O-2(-). Among 106 strains comprising 13 species of enteric gram-negative bacilli and gram-positive cocci, only Lactococcus lactis subspecies lactis produced extracellular O-2(-). Mean (+/-SE) rates of extracellular O-2(-) production in vitro by E. faecalis for isolates associated with bacteremia and endocarditis and for isolates from stool were 2.4+/-0.2, 1.9+/-0.2, and 1.5+/-0.3 nmol of O-2(-) min(-1) 10(9) cfu(-1), respectively (P = .025, analysis of variance), suggesting an association between invasiveness and extracellular O-2(-) production.