From self-assembled bilayer lipid membranes (BLMs) to supported BLMs on metal and gel substrates to practical applications
From self-assembled bilayer lipid membranes (BLMs) to supported BLMs on metal and gel substrates to practical applications
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DOI：
10.1016/s0927-7757(98)00330-6
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发表时间：
1999-04
期刊：
影响因子：
--
通讯作者：
H. Tien;A. Ottova
中科院分区：
文献类型：
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作者：
H. Tien;A. Ottova
毫无疑问，脂质双层研究的灵感来自生物界。自1961年首次报道了体外自组装双层类脂膜，但自1672年罗伯特·胡克时代以来，包括表面、胶体和生物科学家在内的实验科学家一直在研究这些界面现象。BLMS已被广泛应用于从基础膜生物物理研究到通过水光解转换太阳能，再到基于支撑双层类脂膜的生物传感器的开发(S-BLMS)。本文简要总结了本实验室自1974年以来利用BLMS作为某些生物膜模型的研究。此外，我们还详细描述了我们目前在作为实用生物传感器的支撑生物传感器方面的工作。文中还给出了与他人密切合作在S-BLMS上进行的实验。支撑的BLM为各种基于脂质双层的分子传感器奠定了基础，这些传感器是灵敏、通用、廉价(即一次性)的，并且可以进行各种实验。
The inspiration for lipid bilayer research, without question, comes from the biological world. Although the first report on self-assembled bilayer lipid membranes (BLMs) in vitro was reported in 1961, experimental scientists including surface, colloid, and bioscientists have been dealing with these interfacial phenomena since Robert Hooke's time (1672). BLMs have been used in a number of applications ranging from basic membrane biophysics studies to the conversion of solar energy via water photolysis, and to biosensor development using supported bilayer lipid membranes (s-BLMs). This paper briefly summarizes the past research of our laboratory since 1974 on the use of BLMs as models of certain biological membranes. Further, we describe in some details our present work on supported BLMs as practical biosensors. The experiments carried out in close collaboration with others on s-BLMs are also presented. Supported BLMs provide the foundation for a variety of lipid bilayer-based molecular sensors that are sensitive, versatile, inexpensive (i.e. disposable) and open to all sorts of experimentation.