Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin.
Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin.
复制标题
用果蝇 XMAP215、EB1 和 Sentin 重建动态微管。
DOI：
10.1083/jcb.201206101
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发表时间：
2012-11-26
期刊：
影响因子：
--
通讯作者：
Goshima G
中科院分区：
文献类型：
--
作者：
Li W;Moriwaki T;Tani T;Watanabe T;Kaibuchi K;Goshima G
XMAP215msps和EB1-Sentin二人组单独或协同作用，加速微管生长，增加救援事件，但也促进频繁的灾难。动态微管（MTs）是各种细胞内事件，如有丝分裂所必需的。在果蝇S2细胞中，三种MT末端定位蛋白Msps/XMAP215、EB1和Sentin （EB1货物蛋白）已被确定为加速MT生长和促进突变事件的关键蛋白，从而导致动态MT的形成。然而，每种蛋白的分子活性以及这三种因子对MT动态调节的基础尚不清楚。在本文中，我们在体外证明了XMAP215msps在广泛的微管蛋白浓度下具有有效的生长促进活性，而当EB1将Sentin募集到生长中的MT尖端时，会加速生长并增加突变频率。当这三个因素结合在一起时，生长速度协同提高，救援事件最频繁，但频繁的灾难抑制了MT的延长。我们认为，MT动力学是由XMAP215msps聚合酶和EB1-Sentin二人组的独立作用和合作作用促进的。
XMAP215msps and the EB1–Sentin duo act individually and cooperatively to accelerate microtubule growth and increase rescue events but also to promote frequent catastrophes. Dynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs. However, the molecular activity of each protein and the basis of the modulation of MT dynamics by these three factors are unknown. In this paper, we showed in vitro that XMAP215msps had a potent growth-promoting activity at a wide range of tubulin concentrations, whereas Sentin, when recruited by EB1 to the growing MT tip, accelerated growth and also increased catastrophe frequency. When all three factors were combined, the growth rate was synergistically enhanced, and rescue events were observed most frequently, but frequent catastrophes restrained the lengthening of the MTs. We propose that MT dynamics are promoted by the independent as well as the cooperative action of XMAP215msps polymerase and the EB1–Sentin duo.
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DOI：
10.1073/pnas.1014758108
发表时间：
2011-03-08
影响因子：
11.1
作者：
Maurer, Sebastian P.;Bieling, Peter;Surrey, Thomas
通讯作者：
Surrey, Thomas
影响因子：
9.2
作者：
Goshima, G;Wollman, R;Vale, RD
通讯作者：
Vale, RD
影响因子：
64.8
作者：
Kerssemakers, Jacob W. J.;Munteanu, E. Laura;Dogterom, Marileen
通讯作者：
Dogterom, Marileen
影响因子：
2.9
作者：
Manna, Tapas;Honnappa, Srinivas;Wilson, Leslie
通讯作者：
Wilson, Leslie
影响因子：
3.3
作者：
Goshima, G;Vale, RD
通讯作者：
Vale, RD