Expression and crystallographic studies of the D1D2 domains of C4.4A, a homologous protein to the urokinase receptor
Expression and crystallographic studies of the D1D2 domains of C4.4A, a homologous protein to the urokinase receptor
复制标题
C4.4A（尿激酶受体同源蛋白）D1D2 结构域的表达和晶体学研究
DOI：
10.1107/s2053230x17009748
复制
发表时间：
2017
影响因子：
0.9
通讯作者：
Huang Mingdong
中科院分区：
文献类型：
--
作者：
Chen Shanli;Lin Lin;Yuan Cai;Gardsvoll Henrik;Kriegbaum Mette C.;Ploug Michael;Huang Mingdong
C4.4A是一种糖基磷脂酰肌醇锚定的膜蛋白，由两个LU结构域（Ly 6/uPAR样结构域）和一个广泛O-糖基化的C-末端Ser/Thr/Pro富集区组成。C4.4A是一种新的鳞状上皮分化的生物标志物。它的表达在各种病理条件下失调，并且它是各种恶性疾病如肺腺癌中预后不良的稳健生物标志物。为了促进结晶，通过有限的蛋白水解从完整的C4.4A中切除两个LU结构域，通过坐滴气相扩散法纯化并结晶。晶体的衍射分辨率为2.7 nm，空间群为C2221，晶胞参数a = 55.49，B = 119.63，c = 168.54 nm。 统计表明数据质量良好，为C4.4A结构的确定奠定了坚实的基础。
C4.4A is a glycosylphosphatidylinositol-anchored membrane protein comprised of two LU domains (Ly6/uPAR-like domains) and an extensively O-glycosylated C-terminal Ser/Thr/Pro-rich region. C4.4A is a novel biomarker for squamous epithelial differentiation. Its expression is dysregulated under various pathological conditions and it is a robust biomarker for poor prognosis in various malignant conditions such as pulmonary adenocarcinoma. To facilitate crystallization, the two LU domains were excised from intact C4.4A by limited proteolysis, purified and crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to 2.7 Å resolution and belonged to space group C2221, with unit-cell parameters a = 55.49, b = 119.63, c = 168.54 Å. The statistics indicated good quality of the data, which form a solid basis for the determination of the C4.4A structure.