Efficacy and antiparasitic mechanism of 10-gingerol isolated from ginger Zingiber officinale against Ichthyophthirius multifiliis in grass carp
Efficacy and antiparasitic mechanism of 10-gingerol isolated from ginger Zingiber officinale against Ichthyophthirius multifiliis in grass carp
复制标题
生姜10-姜酚对草鱼多子鱼霉的功效及抗寄生虫机制
DOI：
10.1016/j.vetpar.2018.11.011
复制
发表时间：
2019-01-01
影响因子：
2.6
通讯作者：
Guo, Shu-Quan
中科院分区：
文献类型：
--
作者：
Fu, Yao-Wu;Wang, Bin;Guo, Shu-Quan
多子鱼霉是淡水鱼的纤毛虫寄生虫，分布于全球，给水产养殖造成严重的经济损失。本研究旨在研究从生姜中分离出的活性化合物对多子叶螨的功效和抗寄生虫机制。从 Z. officinale 提取物中分离出三种化合物，并鉴定为 10-姜酚、6-脱氢姜烯酚和 6-脱氢-10-姜酚。 10-姜酚在体外表现出最大的抗寄生虫功效。浓度为 2、8 和 16 mg/L 的 10-姜酚分别导致蜂、非包囊和包囊的死亡率为 100%。浓度为 1 mg/L 的 10-姜酚显着降低了鱼体感染性 (p < 0.05)，浓度为 4 mg/L 时可有效治疗受感染的草鱼并保护幼鱼免受多子 I. multifiliis 侵染。其抗寄生虫机制可能与10-姜酚处理后细胞内渗透压升高、自由基积累和细胞膜损伤有关。该研究表明 10-姜酚具有作为针对多子线虫的治疗剂的潜力。
Ichthyophthirius multifiliis is a ciliate parasite of freshwater fish with a global distribution and results in severe economic losses in aquaculture. The present study aimed to investigate the efficacy and antiparasitic mechanism of active compounds isolated from Zingiber officinale against I. multifiliis. Three compounds were isolated from the Z. officinale extract and identified as 10-gingerol, 6-dehydroshogaol, and 6-dehydro-10-gingerol. 10-gingerol demonstrated the greatest antiparasitic efficacy in vitro. 10-gingerol resulted in 100% mortalities of theronts, nonencysted tomonts, and encysted tomonts at concentrations of 2, 8, and 16 mg/L, respectively. 10-gingerol significantly reduced theronts infectivity (p < 0.05) at a concentration of 1 mg/L, and it was effective in treating infected grass carp and protecting naive fish from I. multifiliis infestation at a concentration of 4 mg/L. The antiparasitic mechanism might be attributed to the increase of intracellular osmotic pressure, accumulation of free radicals, and membrane damage of I. multifiliis post 10-gingerol treatment. The study demonstrated that 10-gingerol had the potential as a therapeutic agent against I. multifiliis.