Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions.
Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions.
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DOI：
10.1093/jxb/erw435
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发表时间：
2017-01-01
影响因子：
6.9
通讯作者：
Bernacchi CJ
中科院分区：
文献类型：
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作者：
Köhler IH;Ruiz-Vera UM;VanLoocke A;Thomey ML;Clemente T;Long SP;Ort DR;Bernacchi CJ
在模拟未来气候条件下，结合升高的CO2和升高的温度，在自由空气CO2富集实验中，在大豆中表达蓝藻FBP/SBB 12可防止产量下降。预测表明，到2050年，目前的作物产量需要翻一番，才能满足全球粮食和能源需求。基于理论和实验研究，预期过量表达光合酶景天庚酮糖-1，7-二磷酸酶（SBH 4）可提高C3作物的光合作用和产量。在这里，我们测试如何表达的蓝藻，双功能果糖-1，6/景天庚酮糖-1，7-二磷酸酶（FBP/SBYP）影响碳同化和种子产量（SY）在主要作物（大豆，大豆）。对于三个生长季节，野生型（WT）和FBP/SB酶表达（FS）植物在环境（400 μmol mol−1）和升高（600 μmol mol−1）CO2浓度[CO2]下以及环境和升高的温度（白天+2.7 °C，晚上+3.4 °C）下在ESPRIFACE研究中心的田间生长。在各处理中，FS植物具有显著更高的碳同化（4-14%），Vc，max（5-8%）和Jmax（4-8%）。在环境[CO2]下，升高的温度导致两种基因型的SY显著降低19- 31%。然而，在升高的[CO2]和升高的温度下，FS植物保持SY水平，而WT与单独升高的[CO2]下的植物相比显示出11%至22%的显著降低。这些结果表明，控制光合碳还原循环可以减轻未来高CO2和高温环境对大豆产量的影响。
Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression in a free air CO2 enrichment experiment under simulated future climate conditions with combination of elevated CO2 and elevated temperature. Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 μmol mol−1) and elevated (600 μmol mol−1) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2.7 °C during daytime, +3.4 °C at night) at the SoyFACE research site. Across treatments, FS plants had significantly higher carbon assimilation (4–14%), Vc,max (5–8%), and Jmax (4–8%). Under ambient [CO2], elevated temperature led to significant reductions of SY of both genotypes by 19–31%. However, under elevated [CO2] and elevated temperature, FS plants maintained SY levels, while the WT showed significant reductions between 11% and 22% compared with plants under elevated [CO2] alone. These results show that the manipulation of the photosynthetic carbon reduction cycle can mitigate the effects of future high CO2 and high temperature environments on soybean yield.