Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis–gas chromatography
Characterization of within-tree variation of lignin components in Eucalyptus camaldulensis by pyrolysis–gas chromatography
复制标题
热解-气相色谱法表征赤桉木质素成分的树内变化
作者：
H. Yokoi;Y. Ishida;H. Ohtani;S. Tsuge;T. Sonoda;T. Ona
采用立式微炉裂解装置，采用裂解气相色谱(Py-GC)技术，对赤桉木木素的丁香基/愈创木酰基单元比(S/G比)的“树内变异”进行了精确测定。在450℃下获得的桉树热解图谱上，可重复观察到许多来自木素丁香基和愈创木基的特征峰，以及来自纤维素和半纤维素的特征峰。根据与丁香基和愈创木基单元有关的特征峰的相对强度，仅用约0.1mg木材粉末样品，在约1h内就准确地测定了S/G比值的“树内变化”，相对标准偏差为2%。并将所得结果与硫代酸解方法进行了比较。
Pyrolysis–gas chromatography (Py-GC) using a vertical microfurnace pyrolyzer was applied to the precise determination of the ‘within-tree variation’ of the ratio of syringyl and guaiacyl units (S/G ratio) for lignin in Eucalyptus camaldulensis. On the pyrogram of the Eucalyptus obtained at 450 °C, many characteristic peaks derived from syringyl and guaiacyl units of lignin were reproducibly observed, together with those from cellulose and hemicellulose. On the basis of the relative intensities of those characteristic peaks relating to the syringyl and guaiacyl units, the ‘within-tree variation’ of the S/G ratio was determined precisely in about 1 h using only about 0.1 mg of the powdered wood sample with a 2% relative standard deviation. The results obtained were also compared with those obtained by the thioacidolysis method.