Carbon dots of different composition and surface functionalization: cytotoxicity issues relevant to fluorescence cell imaging
Carbon dots of different composition and surface functionalization: cytotoxicity issues relevant to fluorescence cell imaging
复制标题
DOI：
10.1258/ebm.2011.011132
复制
发表时间：
2011-11-01
影响因子：
3.2
通讯作者：
Sun, Ya-Ping
中科院分区：
文献类型：
--
作者：
Wang, Yanli;Anilkumar, Parambath;Sun, Ya-Ping
纳米级碳颗粒已经成为一类新的类似于半导体量子点的高荧光纳米材料的多功能前体。表面钝化的荧光碳纳米颗粒，被称为‘碳点’，已经在体外和体内展示了它们潜在的光学生物成像应用。在本研究中，我们对前驱体碳纳米颗粒和用于颗粒表面功能化的分子的不同组合制备的碳点进行了系统的细胞毒性评价。结果表明，碳点的细胞毒性与表面钝化分子的选择有关。那些在较高浓度下显示出更显著的细胞毒性的斑点也被评估了它们对活细胞的荧光成像的影响。这一结果对最终将碳点用作细胞显像剂的意义进行了讨论。
Nanoscale carbon particles have emerged as versatile precursors for a new class of highly fluorescent nanomaterials that resemble semiconductor quantum dots. The surface-passivated fluorescent carbon nanoparticles, dubbed 'carbon dots', were already demonstrated for their potential optical bioimaging applications in vitro and in vivo. In this study, we conducted a systematic cytotoxicity evaluation on the carbon dots prepared by various combinations of precursor carbon nanoparticles and molecules for the particle surface functionalization. The results suggested that the cytotoxicity of carbon dots was dependent on the selection of surface passivation molecules. Those dots showing more significant cytotoxicity at higher concentrations were also evaluated for their effects on the fluorescence imaging of live cells. The implications of the results on the eventual use of carbon dots as cell imaging agents are discussed.