Honeybee Kenyon cells are regulated by a tonic GABA receptor conductance.
Honeybee Kenyon cells are regulated by a tonic GABA receptor conductance.
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蜜蜂 Kenyon 细胞受强直 GABA 受体电导调节。
DOI：
10.1152/jn.00180.2014
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发表时间：
2014
影响因子：
2.5
通讯作者：
Palmer MJ
中科院分区：
文献类型：
--
作者：
Palmer MJ
昆虫的高级认知功能依赖于它们的蘑菇体（MB），这在蜜蜂等社会昆虫中特别大。MB Kenyon细胞（KCs）接受多感觉输入，并参与联想学习和记忆。除了通过兴奋性烟碱突触接受感觉输入外，KC还接受来自MB反馈神经元的抑制性GABA能输入。培养的蜜蜂KC表现出离子型GABA受体电流，但完整MB中GABA介导的抑制特性目前尚不清楚。在这里，使用全细胞记录从KC在急性分离的蜜蜂大脑，我们表明，KC表现出紧张电流，抑制印防己毒素，但不是荷包牡丹碱。GABA（5 μM）和牛磺酸（1 mM）的浴用可激活KC中的紧张性电流，但l-谷氨酸（0.1-0.5 mM）无影响。紧张性电流被变构GABA A受体调节剂戊巴比妥强烈增强，并被Cd 2+或硝苯地平抑制Ca 2+通道降低。GABA诱发电流的噪声分析给出了潜在受体的单通道电导值为27 ± 3 pS，与抗狄氏剂（RDL）受体的电导值相似。在印防己毒素存在的情况下，诱发KC动作电位放电所需的注入电流量显着降低。记录在一个完整的蜜蜂头部制备的KC同样表现出紧张性GABA受体电导，降低神经元的兴奋性，一个属性，这是可能有助于在昆虫MB的感觉信息的稀疏编码。
The higher cognitive functions of insects are dependent on their mushroom bodies (MBs), which are particularly large in social insects such as honeybees. MB Kenyon cells (KCs) receive multisensory input and are involved in associative learning and memory. In addition to receiving sensory input via excitatory nicotinic synapses, KCs receive inhibitory GABAergic input from MB feedback neurons. Cultured honeybee KCs exhibit ionotropic GABA receptor currents, but the properties of GABA-mediated inhibition in intact MBs are currently unknown. Here, using whole cell recordings from KCs in acutely isolated honeybee brain, we show that KCs exhibit a tonic current that is inhibited by picrotoxin but not by bicuculline. Bath application of GABA (5 μM) and taurine (1 mM) activate a tonic current in KCs, butl-glutamate (0.1–0.5 mM) has no effect. The tonic current is strongly potentiated by the allosteric GABAAreceptor modulator pentobarbital and is reduced by inhibition of Ca2+channels with Cd2+or nifedipine. Noise analysis of the GABA-evoked current gives a single-channel conductance value for the underlying receptors of 27 ± 3 pS, similar to that of resistant to dieldrin (RDL) receptors. The amount of injected current required to evoke action potential firing in KCs is significantly lower in the presence of picrotoxin. KCs recorded in an intact honeybee head preparation similarly exhibit a tonic GABA receptor conductance that reduces neuronal excitability, a property that is likely to contribute to the sparse coding of sensory information in insect MBs.
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DOI：
--
发表时间：
2004
期刊：
Zeitschrift für Morphologie der Tiere
影响因子：
--
作者：
Dr. W. Witthöft
通讯作者：
Dr. W. Witthöft
蜜蜂大脑蘑菇体的解剖：α-叶的神经连接
影响因子：
2.5
作者：
J. Rybak;R. Menzel
通讯作者：
R. Menzel
影响因子：
2.9
作者：
LEES, G;BEADLE, DJ;BENSON, JA
通讯作者：
BENSON, JA
BIDN 和氟虫腈对果蝇细胞系中稳定表达的果蝇 GABA 受体 (RDL) 的阻断作用的单通道分析
影响因子：
7.3
作者：
F. Grolleau;D. Sattelle
通讯作者：
D. Sattelle
DOI：
--
发表时间：
--
期刊：
影响因子：
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作者：
Run;Delia;BELELLIt;J. Lambertt;John A. PETERSt;A. Reyes;N. Lan
通讯作者：
N. Lan