SEQUENCING OF CORONAVIRUS IBV GENOMIC RNA - 3 OPEN READING FRAMES IN THE 5' UNIQUE REGION OF MESSENGER RNA-D
SEQUENCING OF CORONAVIRUS IBV GENOMIC RNA - 3 OPEN READING FRAMES IN THE 5' UNIQUE REGION OF MESSENGER RNA-D
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DOI：
10.1099/0022-1317-66-10-2253
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
BROWN, TDK
中科院分区：
文献类型：
--
作者：
BOURSNELL, MEG;BINNS, MM;BROWN, TDK
测定了鸡传染性支气管炎病毒（IBV）Beaudette株mRNA D 5′端结构域基因组cDNA克隆的核苷酸序列。该区域包含三个开放阅读框，预测分子量为6700 s（6.7K）、7.4K和12.4K的多肽。预测的12.4K多肽具有与IBV核衣壳、膜和刺突基因产物预测的密码子使用非常相似的密码子使用。该序列还预测了12.4K多肽的N末端一半中的疏水性、潜在的膜锚定区域和亲水性C末端。
The nucleotide sequence of a genomic cDNA clone corresponding to the 5′ terminal domain of mRNA D of the Beaudette strain of infectious bronchitis virus (IBV) has been determined. This region contains three open reading frames which predict polypeptides of molecular weights 6700 s(6.7K), 7.4K and 12.4K. The predicted 12.4K polypeptide has a codon usage very similar to that predicted for the products of the IBV nucleocapsid, membrane and spike genes. The sequence also predicts a hydrophobic, potentially membrane-anchoring, region in the N terminal half of the 12.4K polypeptide, and a hydrophilic C terminus.