THE RELATIONSHIP OF IL-4-PRODUCING AND IFN-GAMMA-PRODUCING T-CELLS STUDIED BY LINEAGE ABLATION OF IL-4-PRODUCING CELLS
THE RELATIONSHIP OF IL-4-PRODUCING AND IFN-GAMMA-PRODUCING T-CELLS STUDIED BY LINEAGE ABLATION OF IL-4-PRODUCING CELLS
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DOI：
10.1016/0092-8674(93)90542-x
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发表时间：
1993-12-03
期刊：
影响因子：
64.5
通讯作者：
FLAVELL, RA
中科院分区：
文献类型：
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作者：
KAMOGAWA, Y;MINASI, LAE;FLAVELL, RA
CD4 T 细胞亚群由它们产生的细胞因子定义；这些细胞因子决定这些细胞的效应功能。克隆的 CD4 T 细胞分为两个亚群，与其他细胞因子结合产生干扰素-γ (IFNgamma) 或白细胞介素-4 (IL-4)，分别称为 Th1 和 Th2 细胞。初始 T 细胞与效应 Th1 型和 Th2 型细胞之间的谱系关系尚不清楚。我们培育了转基因小鼠，其中产生 IL-4 的细胞表达单纯疱疹病毒 1 胸苷激酶，并被更昔洛韦 (GANC) 消除。在 GANC 存在的情况下，转基因 T 细胞的激活消除了 IL-4 和 IFNγ 的产生，表明产生 IL-4 和 IFNγ 的细胞表达或已经表达了 IL-4。这些结果表明，产生IL-4或IFNγ的效应细胞具有共同的前体细胞，其表达IL-4基因。
Subsets of CD4 T cells are defined by the cytokines that they produce; these cytokines determine the effector function of these cells. Cloned CD4 T cells fall into two subsets, producing either interferon-gamma (IFNgamma) or interleukin-4 (IL-4) in combination with other cytokines, and are called Th1 and Th2 cells, respectively. The lineage relationship between naive T cells and effector Th1- and Th2-type cells is unclear. We generated transgenic mice in which IL-4-producing cells express herpes simplex virus 1 thymidine kinase and are eliminated by ganciclovir (GANC). Activation of transgenic T cells in the presence of GANC eliminates IL-4 and IFNgamma production, showing that IL-4- and IFNgamma-producing cells express or have expressed IL-4. These results show that effector cells producing either IL-4 or IFNgamma have a common precursor, which expresses the IL-4 gene.