IMMUNOCYTOCHEMISTRY AND INSITU HYBRIDIZATION OF EPIDERMAL GROWTH-FACTOR RECEPTOR AND RELATION TO PROGNOSTIC FACTORS IN BREAST-CANCER
IMMUNOCYTOCHEMISTRY AND INSITU HYBRIDIZATION OF EPIDERMAL GROWTH-FACTOR RECEPTOR AND RELATION TO PROGNOSTIC FACTORS IN BREAST-CANCER
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DOI：
10.1016/0959-8049(92)90449-c
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发表时间：
1992-01-01
影响因子：
8.4
通讯作者：
MATHIJS, JM
中科院分区：
文献类型：
--
作者：
BILOUS, M;MILLIKEN, J;MATHIJS, JM
通过冰冻切片免疫细胞化学研究了 193 名原发性乳腺癌患者中表皮生长因子受体 (EGFR) 的乳腺肿瘤分布。 EGFR 与 Ki-67 评估的生长分数相关（P = 0.0009），与雌激素受体（ER，P = 0.0001）和孕激素受体（PR，P = 0.0001）状态呈负相关。在 47 名患者中，EGFR mRNA 的原位杂交显示与免疫细胞化学评估的 EGFR 蛋白具有良好的一致性。然而，有一些肿瘤在没有 EGFR 蛋白的情况下可以检测到 EGFR mRNA，并且未观察到 EGFR mRNA 翻译的那些肿瘤的 ER 和 PR 状态之间存在差异。这些差异的原因尚不清楚，但这些发现可能代表了乳腺癌细胞受体差异控制的线索。
The breast tumour distribution of epidermal growth factor receptor (EGFR) was studied in 193 patients with primary breast cancer by immunocytochemistry on frozen sections. EGFR was correlated (P = 0.0009) with growth fraction assessed by Ki-67, and negatively correlated with oestrogen receptor (ER, P = 0.0001) and progesterone receptor (PR, P = 0.0001) status. In 47 patients, in-situ hybridisation for EGFR mRNA showed good agreement with the immunocytochemically assessed EGFR protein. There were, however, several tumours in which EGFR mRNA could be detected in the absence of EGFR protein and there were differences between the ER and PR status of those tumours in which translation of EGFR mRNA was not seen. The cause of these differences is unclear, but these findings may represent a clue as to the differential control of breast cancer cell receptors.