One-Step Fabrication of a Microfluidic Device with an Integrated Membrane and Embedded Reagents by Multimaterial 3D Printing
One-Step Fabrication of a Microfluidic Device with an Integrated Membrane and Embedded Reagents by Multimaterial 3D Printing
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DOI：
10.1021/acs.analchem.7b00409
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发表时间：
2017-04-18
影响因子：
7.4
通讯作者：
Breadmore, Michael C.
中科院分区：
文献类型：
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作者：
Li, Feng;Smejkal, Petr;Breadmore, Michael C.
开发基于微流体的智能传感系统的最大障碍之一是集成复杂设备的可制造性。在这里，我们提出了多材料3D打印在一个步骤中制造这种设备。通过3D打印制作了一种包含集成多孔膜和嵌入式液体试剂的微流控装置，并将其应用于土壤中硝酸盐的分析。集成的，密封的设备的制造实现了作为一个单一的打印在30分钟内。该设备的主体是在透明的丙烯腈丁二烯苯乙烯（ABS）印刷，并包含一个400 μ m宽的结构，从市售的复合材料长丝印刷。通过溶解水溶性材料，可以将复合丝转变成多孔材料。在恢复密封器械之前，通过短暂暂停打印来整合液体试剂。通过测定含有锌颗粒的土壤泥浆中的硝酸盐，使用Griess试剂将硝酸盐还原为亚硝酸盐，对装置进行评价。使用消费者数码相机，检测器响应的线性范围为0至60 ppm，覆盖土壤中硝酸盐的正常范围。为了确保保持试剂室的密封，应避免使用水性试剂。当使用非水试剂时，含有Griess试剂的多材料器械可储存4天以上，但检测范围增加至100500 ppm。多材料3D打印是制造具有多个集成功能组件的微流体设备的潜在新方法。
One of the largest impediments in the development of microfluidic-based smart sensing systems is the manufacturability of integrated, complex devices. Here we propose multimaterial 3D printing for the fabrication of such devices in a single step. A microfluidic device containing an integrated porous membrane and embedded liquid reagents was made by 3D printing and applied for the analysis of nitrate in soil. The manufacture of the integrated, sealed device was realized as a single print within 30 min. The body of the device was printed in transparent acrylonitrile butadiene styrene (ABS) and contained a 400 mu m wide structure printed from a commercially available composite filament. The composite filament can be turned into a porous material through dissolution of a water-soluble material. Liquid reagents were integrated by briefly pausing the printing before resuming for sealing the device. The devices were evaluated by the determination of nitrate in a soil slurry containing zinc particles for the reduction of nitrate to nitrite using the Griess reagent. Using a consumer digital camera, the linear range of the detector response ranged from 0 to 60 ppm, covering the normal range of nitrate in soil. To ensure that the sealing of the reagent chamber is maintained, aqueous reagents should be avoided. When using the nonaqueous reagent, the multimaterial device containing the Griess reagent could be stored for over 4 days but increased the detection range to 100500 ppm. Multimaterial 3D printing is a potentially new approach for the manufacture of microfluidic devices with multiple integrated functional components.