Development of a Class-Specific Competitive ELISA for the Benzoylphenylurea Insecticides
Development of a Class-Specific Competitive ELISA for the Benzoylphenylurea Insecticides
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DOI：
10.1021/jf9800920
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发表时间：
1998-07
影响因子：
6.1
通讯作者：
Shuo Wang;R. Allan;J. Skerritt;I. Kennedy
中科院分区：
文献类型：
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作者：
Shuo Wang;R. Allan;J. Skerritt;I. Kennedy
建立了一种竞争性酶联免疫吸附试验（ELISA），用于土壤（90%甲醇提取液）和水中5种苯甲脲类杀虫剂的定量检测。未观察到显著的基质效应。通过在吡虫脲的脲氮或苯胺环的4-位上连接来制备吡虫脲衍生物与蛋白质的缀合物，其中在半抗原和蛋白质载体之间使用各种长度的桥接基团。检查每种免疫原的抗血清和酶示踪剂的所有组合，并使用通过琥珀酰链在4-苯胺位置偶联的半抗原缀合物的抗体开发测定法。该测定法给出了50%的抗体结合抑制（IC 50），其中，对于灭幼脲为0.6 ppb，对于伏虫脲为5 ppb，对于氟虫脲为10 ppb，对于虱螨脲为31 ppb，对于定虫脲为45 ppb，这些化合物的检测限在0.05 - 2.3 ppb的范围内。DIFL的上级交叉反应的基础是：
A competitive enzyme-linked immunosorbert assay (ELISA) was developed for the quantitative detection of five benzophenylurea insecticides in soil (90% methanol extraction) and water. No significant matrix effects were observed. Conjugates of diflubenzuron derivatives with proteins were prepared by attachment at either the urea nitrogen of diflubenzuron or the 4-position of the aniline ring, wherein bridging groups of various lengths were used between the hapten and the protein carrier. All combinations of antiserum to each immunogen and enzyme tracer were examined, and an assay was developed using an antibody developed to a hapten conjugate coupled through a succinyl chain at the 4-aniline position. The assay gave 50% inhibition of antibody binding (IC50) at 0.6 ppb for diflubenzuron, 5 ppb for teflubenzuron, 10 ppb for flufenoxuron, 31 ppb for lufenuron, and 45 ppb for chlorfluazuron, with detection limits in the range of 0.05−2.3 ppb for these compounds. The basis for the superior cross-reaction of difl...