NON-FLAGELLAR FILAMENTOUS APPENDAGES (FIMBRIAE) AND HAEMAGGLUTINATING ACTIVITY IN BACTERIUM-COLI
NON-FLAGELLAR FILAMENTOUS APPENDAGES (FIMBRIAE) AND HAEMAGGLUTINATING ACTIVITY IN BACTERIUM-COLI
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DOI：
10.1002/path.1700700210
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发表时间：
1955-01-01
期刊：
影响因子：
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通讯作者：
EDMUNDS, PN
中科院分区：
文献类型：
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作者：
DUGUID, JP;SMITH, IW;EDMUNDS, PN
47株B。大肠杆菌进行电镜观察。观察到两种丝状附属物：“鞭毛”，稀少（例如每个芽孢杆菌1-4个），波浪形，长且0.02 [mu]宽，和“菌毛”，大量（例如每个芽孢杆菌100-250个），短且0.01 [mu]宽。33个运动型菌株均出现鞭毛，14个非运动型菌株均无鞭毛，显然是运动器官。33种运动菌株中有25种和14种非运动菌株中有6种的菌毛发生与运动无关;它们的存在与杆菌中的一种力量有关，使它们能够粘附和凝集各种细胞，包括红细胞。47株中有34株具有血凝活性。在37 ℃的蛋白胨水中生长24 - 48小时后，其发育最快。31株表现出大致相似的活动，每个凝集大多数的红细胞品种测试。在所有这31个菌株中均存在菌毛（尽管在使其不凝集的条件下生长的培养物中不存在菌毛）。在13株完全无血凝活性的菌株中均未发现菌毛，在3株特异性较弱的血凝活性菌株中也未发现菌毛。由此得出结论，菌毛的功能是作为将杆菌结合到细胞表面的附着器官，并且它们负责B的常见血凝活性。杆菌
Forty-seven strains of B. coli were examined by the electron micro-scope. Two kinds of filamentous appendages were seen: "flagella", scanty (e.g. 1-4 per bacillus), wavy, long and 0.02 [mu] wide, and "fimbriae", numerous (e.g. 100-250 per bacillus), short and 0.01 [mu] wide. The flagella occurred in all of 33 motile strains and in none of 14 non-motile strains, apparently being the organs of locomotion. The fimbriae occurred independently of motility, in 25 of 33 motile strains and in 6 of 14 non-motile strains; their presence was associated with a power in the bacilli enabling them to adhere to and agglutinate various kinds of cells, including red blood cells. Haemagglutinating activity was observed in 34 of the 47 strains. Its development was maximal after growth in peptone water for 24 - 48 hours at 37[degree]C. Thirty-one strains showed generally similar activities, each agglutinating most of the red-cell varieties tested. Fimbriae were present in all these 31 strains (though not in cultures grown under such conditions as rendered them non-agglutinating.) Fimbriae were not found in any of the 13 strains wholly devoid of hemagglutinating activity, nor in 3 strains which showed weak hemagglutinating activities of unusual specificity. It was concluded that the fimbriae function as organs of attachment which bind the bacilli to cell surfaces, and that they are responsible for the common hemagglutinating activities of B. coli.