Inertial Microfluidics Enabling Clinical Research.
Inertial Microfluidics Enabling Clinical Research.
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DOI：
10.3390/mi12030257
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发表时间：
2021-03-03
期刊：
影响因子：
3.4
通讯作者：
Hur SC
中科院分区：
文献类型：
--
作者：
Kalyan S;Torabi C;Khoo H;Sung HW;Choi SE;Wang W;Treutler B;Kim D;Hur SC
快速准确地分析含有患病细胞或病原体的复杂样本对于就临床和公共卫生问题做出明智的决策非常重要。惯性微流体已越来越多地用于此类研究，以通过基于尺寸和/或变形性的操作从液体样品中分离目标生物颗粒。这种现象对于临床特别有用，因为它具有快速、无标记的靶标富集性质，可以进行进一步的下游测定。惯性微流体利用惯性聚焦原理，依靠颗粒上的惯性力和粘性力的平衡将它们排列成与尺寸相关的层流流线。几种不同的微流体通道几何形状（例如直的、弯曲的、螺旋的、收缩-膨胀阵列）已经过优化，以实现用于各种目的的惯性聚焦，包括颗粒纯化和富集、溶液交换以及用于芯片分析的颗粒对准。在这篇综述中，我们将讨论惯性微流体技术如何有助于提高各种测定的准确性，以提供临床相关信息。这篇全面的综述扩展了对现实世界样本中内源性和外源性目标的研究，重点介绍了具有双重功能的著名混合设备，并对该领域不断发展的前景进行了评论。
Fast and accurate interrogation of complex samples containing diseased cells or pathogens is important to make informed decisions on clinical and public health issues. Inertial microfluidics has been increasingly employed for such investigations to isolate target bioparticles from liquid samples with size and/or deformability-based manipulation. This phenomenon is especially useful for the clinic, owing to its rapid, label-free nature of target enrichment that enables further downstream assays. Inertial microfluidics leverages the principle of inertial focusing, which relies on the balance of inertial and viscous forces on particles to align them into size-dependent laminar streamlines. Several distinct microfluidic channel geometries (e.g., straight, curved, spiral, contraction-expansion array) have been optimized to achieve inertial focusing for a variety of purposes, including particle purification and enrichment, solution exchange, and particle alignment for on-chip assays. In this review, we will discuss how inertial microfluidics technology has contributed to improving accuracy of various assays to provide clinically relevant information. This comprehensive review expands upon studies examining both endogenous and exogenous targets from real-world samples, highlights notable hybrid devices with dual functions, and comments on the evolving outlook of the field.
影响因子：
64.5
作者：
Aceto N;Bardia A;Miyamoto DT;Donaldson MC;Wittner BS;Spencer JA;Yu M;Pely A;Engstrom A;Zhu H;Brannigan BW;Kapur R;Stott SL;Shioda T;Ramaswamy S;Ting DT;Lin CP;Toner M;Haber DA;Maheswaran S
通讯作者：
Maheswaran S
影响因子：
11.5
作者：
Allard, WJ;Matera, J;Terstappen, LWMM
通讯作者：
Terstappen, LWMM