Studies on the selection of new matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Studies on the selection of new matrices for ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
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紫外基质辅助激光解吸/电离飞行时间质谱新基质选择研究。
DOI：
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发表时间：
1996
影响因子：
2
通讯作者：
U. Schlunegger
中科院分区：
文献类型：
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作者：
Joern Krause;Markus Stoeckli;U. Schlunegger
一组新的化合物已被成功地测试为基质的紫外基质辅助激光解吸/电离质谱（UV-MALDI MS）。通过选择在紫外光照射下沿沿着分子内氢键进行分子内质子转移的化合物作为潜在的基质，已经发现了几种新的UV-MALDI MS基质。这种类型的化合物例如是水杨酰胺、水杨酰苯胺、几种邻羟基苯乙酮和邻羟基二苯甲酮。将这些化合物的基质活性与相应的Meta位和对位异构体以及与诸如2，5-二羟基苯甲酸和2，4，6-三羟基苯乙酮等公知基质的基质活性进行比较。发现Meta位和对位取代的羟基羰基化合物与邻位异构体相比显示出显著较低的基质活性或没有基质活性。
A new group of compounds has been successfully tested as matrices for ultraviolet matrix-assisted laser desorption/ionization mass spectrometry (UV-MALDI MS). Several new matrices for UV-MALDI MS have been found by choosing, as potential matrices, compounds that perform an intramolecular proton transfer along an intramolecular H-bond under UV irradiation. Compounds of this type are, for example, salicylamide, salicylanilide, several ortho-hydroxyacetophenones and ortho-hydroxybenzophenones. The matrix activity of these compounds is compared to the corresponding meta- and para-isomers and to the matrix activity of such well known matrices as 2,5-dihydroxybenzoic acid and 2,4,6-trihydroxyacetophenone. It was found that meta- and para-substituted hydroxycarbonyl compounds show either a significantly lower or no matrix activity compared with the ortho isomers.