DEVELOPMENT OF HAEMONCHUS CONTORTUS TO FOURTH STAGE IN VITRO
DEVELOPMENT OF HAEMONCHUS CONTORTUS TO FOURTH STAGE IN VITRO
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DOI：
10.2307/3276403
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发表时间：
1966-01-01
影响因子：
1.3
通讯作者：
SOMMERVILLE, RI
中科院分区：
文献类型：
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作者：
SOMMERVILLE, RI
捻转血矛线虫脱鞘感染幼虫在二氧化碳盐溶液中发育至第四期。形态变化与绵羊蠕虫相似，但发育较慢。最大数量的第四阶段的幼虫，从气体与40%的二氧化碳的解决方案。48小时后出现少量第四阶段蠕虫，72小时后多达90%的蠕虫处于第四阶段。一旦幼虫开始发育，就可以排出二氧化碳，并且在空气中很容易进行发育。在体外培养的第三阶段蠕虫的肠道中未检测到用荧光染料标记的血清，但在第四阶段蠕虫中存在。在此条件下，第三期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第四期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第四期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第四期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第四期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第四期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第四期幼虫的生长发育过程中，第三期幼虫的生长发育过程中，第四期幼虫的生长发育过程中。回旋肌无法摄取培养基。
Ex-sheathed infective larvae of Haemonchus contortus developed to the fourth stage in a salt solution under carbon dioxide. The changes in morphology were similar to those seen in worms from sheep, but development was slower. Greatest numbers of fourth-stage larvae were obtained from solutions gassed with 40% carbon dioxide. Small numbers of fourth-stage worms were present after 48 hr, and as many as 90% were in the fourth stage after 72 hr. Once larvae had commenced to develop carbon dioxide could be withdrawn, and development proceeded readily under air. Serum labeled with a fluorochrome was not detected in the intestine of third-stage worms cultivated in vitro, but was present in fourth-stage worms. It is concluded that under these conditions third-stage larvae of H. contortus were unable to ingest the medium.