EFFECTS OF REPEATED ADMINISTRATION OF DESIPRAMINE OR ELECTROCONVULSIVE SHOCK ON NOREPINEPHRINE UPTAKE SITES MEASURED BY [H-3] NISOXETINE AUTORADIOGRAPHY
EFFECTS OF REPEATED ADMINISTRATION OF DESIPRAMINE OR ELECTROCONVULSIVE SHOCK ON NOREPINEPHRINE UPTAKE SITES MEASURED BY [H-3] NISOXETINE AUTORADIOGRAPHY
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DOI：
10.1016/0006-8993(92)90134-u
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发表时间：
1992-06-12
期刊：
影响因子：
2.9
通讯作者：
TEJANIBUTT, SM
中科院分区：
文献类型：
--
作者：
BAUER, ME;TEJANIBUTT, SM
用放射自显影技术测定重复给予地塞帕明(DMI)或电惊厥(ECS)对去甲肾上腺素(NE)摄取部位的影响。各组动物分别单次注射DMI或每日重复注射10 mg/kg，连续21天，最后一次注射后48h处死动物。另一组大鼠每天接受ECS(150 mA，300ms，60 Hz)，持续12天，在最后一次电击后24小时处死。重复服用DMI导致17个脑区中的8个脑区H-3]NIS的结合量显著降低(20%-40%)；这些脑区包括海马体、丘脑和杏仁核。除杏仁中央外侧核外，DMI的急性处理对所分析的任何区域的[H-3]NIS结合没有影响。相比之下，除了丘脑室旁核，ECS引起的结合略有增加(20%)，其他脑区都没有受到ECS的影响。由此可见，重复给予DMI和慢性ECS处理对[H-3]NIS与大鼠脑内NE摄取部位的结合有不同的影响。
To determine if repeated administration of desipramine (DMI) or electroconvulsive shock (ECS) regulate uptake sites for norepinephrine (NE) in rat brain, the binding of [H-3]nisoxetine ([H-3]NIS) was measured using quantitative autoradiography. Groups of animals were given DMI intraperitoneally, either a single injection or repeated doses of 10 mg/kg once daily for 21 days and were killed 48 h after the last injection. Another group of rats received ECS daily for 12 days (150 mA, 300 ms, 60 Hz) and was killed 24 h after the last shock. Repeated administration of DMI caused statistically significant decreases (20-40%) in the binding of H-3]NIS in 8 out of 17 brain regions measured; these areas included the hippocampus, thalamus and the amygdala. Acute treatment with DMI had no effect on the binding of [H-3]NIS in any of the regions analyzed except the centrolateral nucleus of the amygdala. By contrast, except for the paraventricular nucleus of the thalamus where ECS caused a modest (20%) increase in binding, no other brain region was affected by ECS. Thus it appears that repeated administration of DMI and chronic ECS treatment have different effects on the binding of [H-3]NIS to uptake sites for NE in rat brain.