Wrapping With Basic Fibroblast Growth Factor-Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia
Wrapping With Basic Fibroblast Growth Factor-Impregnated Collagen Sheet Reduces Rat Sciatic Nerve Allodynia
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DOI：
10.1002/jor.24349
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发表时间：
2019-10-01
影响因子：
2.8
通讯作者：
Ohtori, Seiji
中科院分区：
文献类型：
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作者：
Mukai, Michiaki;Uchida, Kentaro;Ohtori, Seiji
自体静脉包裹术用于治疗复发性慢性缩窄性神经病和外伤性周围神经损伤。然而，由于无法获得足够长的静脉用于较大的移植物，其使用受到限制。我们之前报道了静脉源性碱性成纤维细胞生长因子（bFGF）促进血红素加氧酶-1 (HO-1)，通过其抗炎特性减少异位性疼痛。为了模拟静脉包裹，我们研制了一种浸透bFGF的胶原蛋白薄片。以雄性Wistar大鼠作为坐骨神经慢性收缩损伤模型，将大鼠分为三组：(i) CCI手术后未处理组（对照组），（ii）用磷酸盐缓冲盐水浸泡的胶原膜（PBS/CS组），（iii）用bFGF浸泡的胶原膜（bFGF/CS组）。在术后第1、5、7、14天评估疼痛行为（von Frey试验）。对坐骨神经RNA进行定量聚合酶链反应，定量HO-1基因Hmox1的表达。酶联免疫吸附法测定POD 1上HO-1蛋白水平。von Frey试验显示，对照组和PBS/CS组疼痛超敏反应明显高于bFGF/CS组。bFGF/CS组坐骨神经中Hmox1信使RNA和HO-1蛋白水平分别在POD 1、POD 5和POD 1上显著高于对照组和PBS/CS组。经静脉包裹观察，bFGF/CS组异常性疼痛和HO-1诱导减少。因此，局部应用bFGF可能是临床治疗压迫性神经病变和周围神经损伤的一种替代治疗策略。(c) 2019年骨科研究学会。Wiley期刊公司出版。[J]中华骨科杂志，2019,37 (2):558 - 563
Autologous vein wrapping is used to treat recurrent chronic constriction neuropathy and traumatic peripheral nerve injury. However, its use is restricted due to the inability to obtain sufficiently long veins for larger grafts. We previously reported that vein-derived basic fibroblast growth factor (bFGF) promotes heme oxygenase-1 (HO-1), which reduces allodynia via its anti-inflammatory properties. To mimic vein wrapping, we developed a collagen sheet impregnated with bFGF. Chronic constriction injury (CCI) was induced in male Wistar rats as a model of sciatic nerve injury, and the rats were divided into three groups: (i) untreated after CCI surgery (control group), (ii) treated with a collagen sheet wrap impregnated with phosphate-buffered saline (PBS/CS group), and (iii) treated with a collagen sheet wrap impregnated with bFGF (bFGF/CS group). Pain behavior (von Frey test) was evaluated on postoperative days (PODs) 1, 5, 7, and 14. Quantitative polymerase chain reaction was conducted on sciatic nerve RNA to quantify HO-1 gene, Hmox1, expression. Enzyme-linked immunosorbent assay were used to determine HO-1 protein levels on POD 1. von Frey testing showed significantly greater pain hypersensitivity in the control and PBS/CS groups than the bFGF/CS group. In the bFGF/CS group, Hmox1 messenger RNA and HO-1 protein levels were significantly increased in the sciatic nerve compared with the control and PBS/CS groups on PODs 1 and 5 and POD 1, respectively. The bFGF/CS group showed decreased allodynia and HO-1 induction, as observed with vein wrapping. Therefore, local application of bFGF may be an alternative treatment strategy for compressive neuropathy and peripheral nerve trauma in clinical settings. (c) 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2258-2263, 2019