A spatially resolved single-cell genomic atlas of the adult human breast
A spatially resolved single-cell genomic atlas of the adult human breast
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DOI：
10.1038/s41586-023-06252-9
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发表时间：
2023-06-28
期刊：
影响因子：
64.8
通讯作者：
Navin, Nicholas
中科院分区：
文献类型：
--
作者：
Kumar, Tapsi;Nee, Kevin;Navin, Nicholas
成人乳腺由上皮导管和小叶组成的复杂网络组成，这些导管和小叶嵌入结缔组织和脂肪组织中（1-3）。虽然大多数以前的研究集中在乳腺上皮系统（4-6），许多非上皮细胞类型仍然研究不足。在这里，我们以单细胞和空间分辨率构建了全面的人类乳腺细胞图谱（HBCA）。我们的单细胞转录组学研究分析了来自126名女性的714，331个细胞和来自20名女性的117，346个细胞核，确定了12种主要细胞类型和58种生物细胞状态。这些数据揭示了丰富的血管周围，内皮和免疫细胞群，和高度多样化的管腔上皮细胞状态。使用四种不同技术的空间映射揭示了组织驻留免疫细胞的意外丰富的生态系统，以及导管和小叶区域之间的明显分子差异。这些数据为研究乳腺生物学和乳腺癌等疾病提供了成人正常乳腺组织的参考。
The adult human breast is comprised of an intricate network of epithelial ducts and lobules that are embedded in connective and adipose tissue(1-3). Although most previous studies have focused on the breast epithelial system(4-6), many of the non-epithelial cell types remain understudied. Here we constructed the comprehensive Human Breast Cell Atlas (HBCA) at single-cell and spatial resolution. Our single-cell transcriptomics study profiled 714,331 cells from 126 women, and 117,346 nuclei from 20 women, identifying 12 major cell types and 58 biological cell states. These data reveal abundant perivascular, endothelial and immune cell populations, and highly diverse luminal epithelial cell states. Spatial mapping using four different technologies revealed an unexpectedly rich ecosystem of tissue-resident immune cells, as well as distinct molecular differences between ductal and lobular regions. Collectively, these data provide a reference of the adult normal breast tissue for studying mammary biology and diseases such as breast cancer.