Apparent lack of neutralizing antibodies in Aleutian disease is due to masking of antigenic sites by phospholipids.
Apparent lack of neutralizing antibodies in Aleutian disease is due to masking of antigenic sites by phospholipids.
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阿留申病中明显缺乏中和抗体是由于磷脂掩盖了抗原位点。
DOI：
10.1016/0042-6822(87)90251-0
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发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
O. Kaaden
中科院分区：
文献类型：
--
作者：
B. Stolze;O. Kaaden
阿留申病病毒（ADV）在体内和体外均不能被抗体中和。我们发现了几种方法来证明水貂特异性抗体对ADV的中和作用。在中和试验前，用月桂酰肌氨酸钠或正丁醇处理或通过0.05 μm膜过滤使ADV单分散是必要的。在动力学实验中，在反应的前5分钟内，病毒感染性损失95%，但孵育1.5小时后仍有约1%的抗性分数。在自然感染和实验感染的水貂血清中，中和滴度在1：160和1：640之间。中和和ELISA滴度之间始终存在正相关。此外，通过丁醇提取的病毒体的薄层色谱显示磷脂与ADV的分离。通过用各种脂质重构单分散的ADV，发现磷脂通过附着于病毒表面而干扰病毒中和。
It is generally accepted that Aleutian disease virus (ADV) cannot be neutralized by antibodies eitherin vivoorin vitro. We found several ways to demonstrate neutralization of ADV by specific antibodies from mink. It was essential to make ADV monodisperse by treatment with sodium lauroyl sarkosyl orn-butanol or by filtration through 0.05-μm membranes before neutralization tests. In kinetic experiments, there was a 95% loss of virus infectivity within the first 5 min of reaction, but a resistant fraction of about 1% remained after 1.5 hr of incubation. Neutralization titers between 1:160 and 1:640 were found in sera from naturally and experimentally infected mink. A positive relation was consistently found between neutralization and ELISA titers. Furthermore, separation of phospholipids from ADV was shown by thin-layer chromatography of butanol-extracted virions. By reconstitution of monodispersed ADV with various lipids, phospholipids were found to interfere with virus neutralization by attachment to the virus surface.
水貂阿留申病增加的IgG大部分是病毒抗体。
DOI：
--
发表时间：
1984
期刊：
Journal of experimental pathology
影响因子：
--
作者：
Porter,DD;Porter,HG;Larsen,AE
通讯作者：
Larsen,AE
阿留申病：水貂的一种持续性细小病毒感染，具有最大但无效的宿主体液免疫反应。
DOI：
--
发表时间：
1986
期刊：
Progress in medical virology. Fortschritte der medizinischen Virusforschung. Progres en virologie medicale
影响因子：
--
作者：
Porter,DD
通讯作者：
Porter,DD