Outbreak of Marek's disease in a vaccinated broiler breeding flock during its peak egg-laying period in China.
Outbreak of Marek's disease in a vaccinated broiler breeding flock during its peak egg-laying period in China.
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中国产蛋高峰期接种疫苗的肉鸡种鸡群中爆发马立克氏病
DOI：
10.1186/s12917-015-0493-7
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发表时间：
2015-07-23
影响因子：
2.6
通讯作者：
Qin A
中科院分区：
文献类型：
--
作者：
Zhuang X;Zou H;Shi H;Shao H;Ye J;Miao J;Wu G;Qin A
由马立克病病毒（MDV）引起的马立克病（MD）暴发主要发生在10 - 12周龄的母鸡中。我们报告了一例在中国24 - 30周龄接种疫苗的肉鸡中发生的MD病例。感染鸡的临床症状出现在24周龄，肿瘤发生率在30周龄达到高峰。母鸡的发病率和死亡率分别为5%和80%。苏木精-伊红染色显示出典型的MD特征。用琼脂扩散沉淀法检测鸡羽毛毛囊上皮的MD抗原，证实了MDV感染。从血淋巴细胞中分离出一株MDV，命名为AH1410。pp38、meq和gB基因的序列分析显示，菌株AH1410具有与先前鉴定的强毒性MDV一致的分子特征。我们的数据提供的证据表明，MDV不仅毒性越来越强，而且在鸡中发病的时间也在延长。这些发现为中国MDV毒株的分子监测和进一步研究以及MD的控制策略提供了基础。
Outbreaks of Marek’s disease (MD), caused by Marek’s disease virus (MDV), primarily occur in 10–12-week-old hens. We report a case of MD in a breeding flock of 24–30-week-old vaccinated broilers in China. The clinical signs in the affected chickens appeared at 24 weeks, and the incidence of tumours peaked at 30 weeks. The morbidity and mortality of the hens were 5 % and 80 %, respectively. Hematoxylin–eosin staining of the tissues showed the typical characteristics of MD. MDV infection was confirmed in the hens with an agar gel diffusion precipitation assay for the MD antigen in the feather follicle epithelium. An MDV strain, designated AH1410, was isolated from the blood lymphocytes. Sequence analyses of the pp38, meq, and gB genes revealed that strain AH1410 had molecular features consistent with a virulent, previously identified MDV. Our data provide evidence that not only is MDV becoming more virulent, but that the period of its onset in chickens is expanding. These findings provide the basis the molecular surveillance and further study of virulent MDV mutants and control strategies for MD in China.