Impact of Thiopurine Exposure on Immunogenicity to Infliximab Is Negligible in the Setting of Elevated Infliximab Concentrations.
Impact of Thiopurine Exposure on Immunogenicity to Infliximab Is Negligible in the Setting of Elevated Infliximab Concentrations.
复制标题
在英夫利昔单抗浓度升高的情况下，硫嘌呤暴露对英夫利昔单抗免疫原性的影响可以忽略不计。
DOI：
10.1093/ibd/izab232
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发表时间：
2022
影响因子：
4.9
通讯作者：
Dervieux,Thierry
中科院分区：
文献类型：
--
作者：
Ungaro,RyanC;Colombel,Jean-Frederic;Dubinsky,MarlaC;Jain,Anjali;Dervieux,Thierry
方法我们询问了一个大型商业实验室数据库（普罗米修斯实验室，圣地亚哥，加利福尼亚州）与治疗药物监测（TDM）数据的硫嘌呤（硫唑嘌呤，6-巯基嘌呤）和IFX。包括IFX和ATI检测后30天内治疗医生要求的所有6-硫代鸟嘌呤（6-TGN）水平。使用反相液相色谱法结合UV检测，从红细胞（RBC）裂解物中测定6-TGN水平;使用耐药均相迁移率变动测定法测定IFX和ATI浓度（存档数据，Prometheus Laboratories）。
MethodsWe interrogated a large commercial laboratory database (Prometheus Laboratories, San Diego, CA) with therapeutic drug monitoring (TDM) data on thiopurines (azathioprine, 6-mercaptopurine) and IFX. All 6-thioguanine (6-TGN) levels requested by treating physicians within 30 days of IFX and ATI testing were included. The 6-TGN levels were determined from red blood cell (RBC) lysates using reversed phase liquid chromatography coupled with UV detection; IFX and ATI concentrations were determined using drug-tolerant homogenous mobility shift assays (data on file, Prometheus Laboratories).