IDENTIFICATION AND QUANTIFICATION OF 1,2,3,4-TETRAHYDRO-BETA-CARBOLINE, 2-METHYL-1,2,3,4-TETRAHYDRO-BETA-CARBOLINE, AND 6-METHOXY-1,2,3,4-TETRAHYDRO-BETA-CARBOLINE AS INVIVO CONSTITUENTS OF RAT-BRAIN AND ADRENAL-GLAND
IDENTIFICATION AND QUANTIFICATION OF 1,2,3,4-TETRAHYDRO-BETA-CARBOLINE, 2-METHYL-1,2,3,4-TETRAHYDRO-BETA-CARBOLINE, AND 6-METHOXY-1,2,3,4-TETRAHYDRO-BETA-CARBOLINE AS INVIVO CONSTITUENTS OF RAT-BRAIN AND ADRENAL-GLAND
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DOI：
10.1016/0006-2952(81)90278-1
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发表时间：
1981-01-01
影响因子：
5.8
通讯作者：
CHRISTIAN, ST
中科院分区：
文献类型：
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作者：
BARKER, SA;HARRISON, REW;CHRISTIAN, ST
三种1，2，3，4-四氢-β-本文报道了用气相色谱/质谱联用技术测定大鼠脑和肾上腺正常组织中的咔啉类化合物。组织的定性分析导致鉴定出1，2，3，4-四氢-β-咔啉（THBC）、2-甲基-THBC（2-MTHBC）和6-甲氧基-THBC（6-MeOTHBC），如通过观察到的峰保留时间、质量碎片和离子质量比测定的。使用氘化内标进行定量分析，得出以下结果：大脑中的THBC（ng/g湿重[重量]）= 17.5。+-。4.86，肾上腺= 500.3 ±。163. 6-脑中MeOTHBC（ng/g湿重）= 35.6 .+-。16.6，肾上腺= 1113.7。300.形成这些β-并讨论了咔啉类化合物在体内的可能作用。
The identification and quantification of three 1,2,3,4-tetrahydro-.beta.-carbolines as normal constitutents of rat brain and adrenal gland, using combined gas chromatographic/mass spectrometric techniques, are reported. Qualitative analyses of the tissues led to the identification of 1,2,3,4-tetrahydro-.beta.-carboline (THBC), 2-methyl-THBC (2-MTHBC) and 6-methoxy-THBC (6-MeOTHBC), as determined by observed peak retention times, mass fragments and ion mass ratios. Quantitative analyses, using deuterated internal standards, gave the following results: THBC (ng/g wet wt [weight]) in brain = 17.5 .+-. 4.86, adrenal = 500.3 .+-. 163. 6-MeOTHBC (ng/g wet wt) in brain = 35.6 .+-. 16.6, adrenal = 1113.7 .+-. 300. Mechanisms for the formation of these .beta.-carbolines and their possible function in vivo are discussed.