The role of glycocalyx in surface phagocytosis of Bacteroides spp., in the presence and absence of clindamycin.
The role of glycocalyx in surface phagocytosis of Bacteroides spp., in the presence and absence of clindamycin.
复制标题
在克林霉素存在和不存在的情况下，糖萼在拟杆菌表面吞噬作用中的作用。
DOI：
10.1093/jac/23.5.711
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发表时间：
1989
影响因子：
5.2
通讯作者：
J. Verhoef
中科院分区：
文献类型：
--
作者：
E. Veringa;D. A. Ferguson;D. Lambe;J. Verhoef
多形拟杆菌和B.研究了fragilis对克林霉素处理和未处理的同源和异源物种的表面吞噬作用的影响。当同源或异源分离的糖萼被添加到克林霉素处理的B。或B。在与PMNL孵育之前，将glycocalyx加入到fragilis中，吞噬作用降低到未处理的对照细菌中观察到的水平，但向未处理的对照菌株中加入glycocalyx未显示吞噬作用的降低。将分离的类杆菌-糖萼加入到金黄色葡萄球菌或S.在表皮中，克林霉素处理的和未处理的细菌的吞噬作用均显著降低。分离的glycocalyx制剂进行了分析，通过薄层和气液色谱法，这些制剂是免费的脂多糖。分离的糖萼不影响PMNL活力。我们的研究结果表明，糖萼是一个重要的毒力因子，因为它削弱了类杆菌的吞噬作用。在PMNL。克林霉素可能通过改变糖萼来增强类杆菌的调理吞噬作用。
The influence of isolated glycocalyx from Bacteroides thetaiotaomicron and B. fragilis on surface phagocytosis of clindamycin-treated and -untreated homologous and heterologous species was studied. When homologous or heterologous isolated glycocalyx was added to clindamycin-treated B. thetaiotaomicron or B. fragilis before incubation with PMNL, phagocytosis was reduced to levels observed in the untreated control bacteria, but addition of glycocalyx to untreated control strains showed no reduction of phagocytosis. When isolated bacteroides-glycocalyx was added to Staphylococcus aureus or S. epidermidis, phagocytosis of both clindamycin-treated and -untreated bacteria was significantly reduced. The isolated glycocalyx preparations were analysed by thin layer and gas-liquid chromatography; these preparations were free of lipopolysaccharides. The isolated glycocalyx did not affect PMNL viability. Our findings suggest that the glycocalyx is an important virulence factor because it impairs phagocytosis of Bacteroides spp. by PMNL. Clindamycin may enhance opsonophagocytosis of bacteroides by altering the glycocalyx.