MOLECULAR-WEIGHT ESTIMATIONS OF PROTEINS BY ELECTROPHORESIS IN POLYACRYLAMIDE GELS OF GRADED POROSITY
MOLECULAR-WEIGHT ESTIMATIONS OF PROTEINS BY ELECTROPHORESIS IN POLYACRYLAMIDE GELS OF GRADED POROSITY
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DOI：
10.1016/0014-5793(72)80793-2
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发表时间：
1972-01-01
期刊：
影响因子：
3.5
通讯作者：
MIKAELSSON, M
中科院分区：
文献类型：
--
作者：
ANDERSSON, LO;BORG, H;MIKAELSSON, M
聚丙烯酰胺凝胶电泳已被广泛用作蛋白质分离和表征的技术。实现的分离取决于蛋白质的大小和电荷。许多工作者试图消除电荷效应[1 -7]，从而获得蛋白质分子大小的直接估计。一种方法使用含有阴离子去污剂的缓冲液[1，3，7]，该去污剂与蛋白质结合，使得它们获得相似的电荷，并且pH主要取决于大小。然而，使用这种方法，去污剂与各种蛋白质的结合可能存在变化，并且在某些情况下值可能是错误的。本文描述了一种基于“微孔电泳”的蛋白质和蛋白质亚基的分子量估算方法--在具有梯度孔隙度的聚丙烯酰胺凝胶中进行电泳[8，9]。
Polyacrylamide gel electrophoresis has been extensively used as a technique for the separation and characterization of proteins. The separation achieved is dependent on both the size and charge of the protein. Many workers have attempted to eliminate the charge effect [l-7] and thereby obtain a direct estimate of the size of the protein molecules. One method uses a buffer contai~ ng an anionic detergent [1, 3, 7] which is bound to the proteins so that they acquire similar charges and~ p~ ation is primarily dependent on size. With this method there may however be variations in the binding of detergent to various proteins and values may be erroneous in certain cases. The present paper describes a method for molecular weight estimations of proteins and protein subunits based on “Gradipore electrophoresis”~ electrophoresis in polyacrylamide gels of graded porosity [8, 9].