Physiological and genetic analysis of the glucose-fructose permeation system in two Synechocystis species
Physiological and genetic analysis of the glucose-fructose permeation system in two Synechocystis species
复制标题
两种集胞藻葡萄糖-果糖渗透系统的生理和遗传分析
DOI：
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发表时间：
1988
影响因子：
2.8
通讯作者：
R. Jeanjean
中科院分区：
文献类型：
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作者：
F. Joset;T. Buchou;C.;R. Jeanjean
果糖对集胞藻PCC 6714和6803是有毒的，集胞藻PCC 6714和6803是在葡萄糖上化学异养生长的菌株。这种毒性，以及果糖摄取，抑制葡萄糖或其非代谢类似物3-O-甲基葡萄糖。结果表明，这两种糖是由相同的渗透系统，果糖的亲和力，估计从相应的Km和Ki，是非常低的。通过分离显示预期多效性表型Glu-、Frur-、转运-的自发突变体，并通过在野生型DNA转化的突变体细胞中同时重新获得相关野生型特征，进一步确立了渗透系统的单一性。鉴于不可能从运输缺陷突变体中分离自发逆转的野生型克隆，讨论了这种突变的遗传性质。
Fructose is toxic for Synechocystis PCC 6714 and 6803, strains which grow chemoheterotrophically on glucose. This toxicity, as well as fructose uptake, were inhibited by glucose or by its non-metabolized analogue 3-O-methyl-glucose. The results suggested that both sugars were transported by the same permeation system, the affinity for fructose, estimated from the corresponding Km and Ki, being very low. The unicity of the permeation system was further established by the isolation of spontaneous mutants showing the expected pleiotropic phenotype, Glu−, Frur, transport−, and by the simultaneous re-acquisition of the relevant wild type characteristics in mutant cells transformed by wild type DNA. The genetic nature of this mutation is discussed in view of the impossibility to isolate spontaneously reversed wild type clones from the transport deficient mutants.