TETRAHYMENA HISTONE H-3 - PURIFICATION AND 2 VARIANT SEQUENCES
TETRAHYMENA HISTONE H-3 - PURIFICATION AND 2 VARIANT SEQUENCES
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DOI：
10.1093/oxfordjournals.jbchem.a134788
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
IWAI, K
中科院分区：
文献类型：
--
作者：
HAYASHI, T;HAYASHI, H;IWAI, K
原生动物T.如前所述获得梨形藻（Fusauchi，Y. et Iwai，K.（1983）），并在还原和羧甲基化后通过Sephadex G-50色谱进一步纯化。纯化的H3由2种变体组成，75摩尔%的H3（1）和25摩尔%的H3（2）。H3混合物通过Edman降解从N-末端至残基104直接测序。用来自H3混合物的胰蛋白酶肽和溴化氰片段进一步进行序列测定。完全确定了H3（1）和H3（2）的总序列;两者均由总共135个氨基酸残基组成（H3（1）的未修饰形式的MW为15，336，H3（2）的未修饰形式的MW为15，424）。两者在相同的6个赖氨酸残基处以相似的程度部分乙酰化或甲基化。H3（1）和H3（2）序列在14个位置上彼此不同，并且在17和21个位置上与人脾H3的那些不同（Ohe，Y. et Iwai，K.（1981））。这些结果的影响，这组蛋白物种的结构-功能关系，也为原生动物的繁殖进行了讨论。
The H3 histone of the protozoan T. pyriformis was obtained as described previously (Fusauchi, Y. et Iwai, K. (1983)) and further purified by Sephadex G-50 chromatography after reduction and carboxymethylation. The purified H3 was composed of 2 variants, 75 mol% of H3(1) and 25 mol% of H3(2). The H3 mixture was directly sequenced by Edman degradation from the N-terminal through residue 104. Sequence determination was further performed with tryptic peptides and cyanogen bromide fragments derived from the H3 mixture. The total sequences of H3(1) and H3(2) were completely determined; both consist of a total of 135 amino acid residues (the MW in the unmodified form are 15,336 for H3(1) and 15,424 for H3(2)). Both are partially acetylated or methylated at the same 6 lysine residues to similar extents. The H3(1) and H3(2) sequences differ in 14 positions from each other, and in 17 and 21 positions from those of human spleen H3 (Ohe, Y. et Iwai, K. (1981)). The implications of these results for the structure-function relationship of this histone species and also for the phylogeny of protozoa are discussed.