Comparison of [14C]glucose and [14C]deoxyglucose as tracers of brain glucose use.
Comparison of [14C]glucose and [14C]deoxyglucose as tracers of brain glucose use.
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[14C]葡萄糖和[14C]脱氧葡萄糖作为脑葡萄糖使用示踪剂的比较。
DOI：
10.1152/ajpendo.1988.254.3.e310
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
DeJoseph,MR
中科院分区：
文献类型：
--
作者：
Hawkins,RA;Mans,AM;Davis,DW;DeJoseph,MR
因为葡萄糖代谢和脑区域的功能活动通常是耦合的，所以区域脑葡萄糖使用的知识可以产生对区域功能活动的见解。脱氧葡萄糖（DG）方法被广泛用于实验动物和人类，但其局限性和准确性的问题已经出现。因此，设计了一个实验，在结构的基础上比较DG方法与正常大鼠中葡萄糖使用的另一种示踪剂[6- 14 C]葡萄糖。使用两种示踪剂获得的脑代谢率在端脑中相似，但使用DG的结果在中脑和后脑中显著较低（间脑，18%;中脑，20%;后脑，29%;和中脑，35%）。发现主要DG代谢物DG 6-磷酸（DG-6-P）以不均匀的方式从主要脑结构中消失：端脑小于间脑小于中脑=后脑小于中脑。因此，发现DG-6-P损失率与DG方法提供较低葡萄糖使用值的程度之间存在相关性。因此，这至少可以部分解释两种方法之间的差异。
Because glucose metabolism and functional activity in brain regions are normally coupled, knowledge of regional brain glucose use can yield insights into regional functional activity. The deoxyglucose (DG) method is widely used for this purpose in experimental animals and humans but questions have arisen regarding its limits and accuracy. Therefore an experiment was designed to compare the DG method on a structure-by-structure basis with another tracer of glucose use, [6-14C]glucose, in normal rats. The cerebral metabolic rates obtained using the two tracers were similar in the telencephalon, but the results using DG were substantially lower in the midbrain and hindbrain (diencephalon, 18%; mesencephalon, 20%; metencephalon, 29%; and myelencephalon, 35%). The primary DG metabolite, DG 6-phosphate (DG-6-P) was found to disappear in a non-uniform manner from the major brain structures: telencephalon less than diencephalon less than mesencephalon = metencephalon less than myelencephalon. Thus a correlation was found between the rate of DG-6-P loss and the extent to which the DG method gave lower values of glucose use. Thus this may explain, at least in part, the discrepancies between the two methods.