Lysozymes: characteristics, mechanism of action and technological applications on the control of pathogenic microorganisms
Lysozymes: characteristics, mechanism of action and technological applications on the control of pathogenic microorganisms
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DOI：
10.18781/r.mex.fit.2005-6
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Bautista-Baños, Silvia
中科院分区：
文献类型：
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作者：
Gálvez-Iriqui, Alma Carolina;Plascencia-Jatomea, Maribel;Bautista-Baños, Silvia
由于溶菌酶和类似酶的溶菌酶具有抗菌和抗真菌活性，它们作为控制病原微生物的新替代品正受到人们的关注。虽然在食品工业和作物保护中已经提出了抗菌酶，但它们的使用存在一些缺点。这些问题包括抑制剂、化学物质或加工条件的失活，生产和纯化成本高，以及溶解性和不稳定性问题。酶固定化是一种很有前途的工具，能够减少一些不便，主要是当它打算在固体介质中工作时。本文综述了溶菌酶抗细菌和真菌活性的研究现状，重点介绍了溶菌酶的作用机理。讨论了抗菌溶菌酶最重要的方面和特性及其可能的技术应用，包括壳聚糖基聚合物基质在抗菌酶固定化中的应用。
Since lysozymes and enzymes-like lysozymes have antibacterial and antifungal activity, they are becoming attractive as new alternatives for controlling pathogenic microorganisms. Although the antimicrobial enzymes have been proposed in the food industry and crop protection, their use involves some disadvantages. These include the inactivation by inhibitors, chemicals, or processing conditions, the high production and purification costs, and problems of solubility and instability. Enzyme immobilization is a promising tool capable of reducing some inconveniences, mainly when it is intended to work in solid media. This review shows state of the art on the lysozyme activity against bacteria and fungi, focusing on its mechanisms of action. The most important aspects and characteristics of the antimicrobial lysozymes and their possible technological application, including the application of chitosan-based polymeric matrices for the immobilization of the antimicrobial enzymes, are also discussed.