Cotranslational assembly of the yeast SET1C histone methyltransferase complex
Cotranslational assembly of the yeast SET1C histone methyltransferase complex
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DOI：
10.1038/emboj.2009.240
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发表时间：
2009-10-07
期刊：
影响因子：
11.4
通讯作者：
Dichtl, Bernhard
中科院分区：
文献类型：
--
作者：
Halbach, Andre;Zhang, Haidi;Dichtl, Bernhard
在探索RNA在SET1C/COMPASS组蛋白甲基转移酶功能中的作用时，我们确定了SET1RC(BAR NA相关复合体下的SET1 m(R))，它包含SET1 mRNA和组成SET1C的8个多肽中的4个--Set1、Swd1、Spp1和Shg1。对SET1RC的鉴定表明，SET1 mRNA结合不需要相关的Swd1、Spp1和Shg1蛋白或Set1中存在的RNA识别基序。当Set1蛋白和SET1 mRNA来自独立的基因或当SET1转录物被限制在细胞核中时，没有观察到RNA结合。重要的是，蛋白质-RNA的相互作用对EDTA、翻译延伸抑制剂嘌呤霉素和prt1-1突变体中翻译起始的抑制敏感。综上所述，我们的结果支持这样的观点，即SET1 mRNA的结合依赖于翻译，并且SET1RC以共翻译的方式组装在新生的Set1上。此外，我们发现Set1的细胞积累受到某些SET1C成分的可获得性的限制，如Swd1和Swd3，并提示共翻译蛋白相互作用可能在保护新生Set1免受降解方面发挥作用。EMBO期刊(2009)28,2959-2970。DOI：10.1038/Intemj.2009.240；2009年8月27日在线发布
While probing the role of RNA for the function of SET1C/COMPASS histone methyltransferase, we identified SET1RC (SET1 m (R) under bar NA-associated complex), a complex that contains SET1 mRNA and Set1, Swd1, Spp1 and Shg1, four of the eight polypeptides that constitute SET1C. Characterization of SET1RC showed that SET1 mRNA binding did not require associated Swd1, Spp1 and Shg1 proteins or RNA recognition motifs present in Set1. RNA binding was not observed when Set1 protein and SET1 mRNA were derived from independent genes or when SET1 transcripts were restricted to the nucleus. Importantly, the protein-RNA interaction was sensitive to EDTA, to the translation elongation inhibitor puromycin and to the inhibition of translation initiation in prt1-1 mutants. Taken together, our results support the idea that SET1 mRNA binding was dependent on translation and that SET1RC assembled on nascent Set1 in a cotranslational manner. Moreover, we show that cellular accumulation of Set1 is limited by the availability of certain SET1C components, such as Swd1 and Swd3, and suggest that cotranslational protein interactions may exert an effect in the protection of nascent Set1 from degradation. The EMBO Journal (2009) 28, 2959-2970. doi: 10.1038/emboj.2009.240; Published online 27 August 2009