Preparation and Characterization of Protein-Loaded Electrospun Fiber Mat and Its Release Kinetics.
Preparation and Characterization of Protein-Loaded Electrospun Fiber Mat and Its Release Kinetics.
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DOI：
10.1021/acs.jafc.7b01830
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发表时间：
2017-06
影响因子：
6.1
通讯作者：
Peng Wen;Yan Wen;Xiao Huang;Min-hua Zong;Hong Wu
中科院分区：
文献类型：
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作者：
Peng Wen;Yan Wen;Xiao Huang;Min-hua Zong;Hong Wu
为了提高蛋白质的生物利用度，利用同轴静电纺丝技术研制了一种特定的给药系统。以牛血清白蛋白(BSA)为蛋白质模型，制备了以海藻酸钠为壳层、BSA负载壳聚糖纳米粒子为核心层的核鞘纤维毡。通过优化电纺工艺参数，制得了直径在200~600 nm之间的均匀纤维，并用透射电子显微镜和激光共聚焦扫描显微镜观察了其芯鞘结构。傅里叶变换红外光谱(FTIR)分析表明，各组分之间存在分子相互作用，从而提高了板坯的热稳定性和力学性能。FTIR和圆二色谱分析表明，BSA从纤维毡中释放的主要机理是侵蚀，包埋后的BSA二级结构变化不大。研究表明，所制得的纤维毡是一种潜在的蛋白质递送系统。
For the enhancement of protein's bioavailability, a specific delivery system was developed by coaxial electrospinning. Bovine serum albumin (BSA) was used as protein model, and the core-sheath fiber mat was fabricated using sodium alginate as shell layer and the BSA-loaded chitosan nanoparticle that was prepared previously as core layer. By optimizing electrospinning parameters, uniform fibers with diameters ranging from 200-600 nm were obtained, and transmission electron microscopy and confocal laser scanning microscopy revealed their core-sheath structures. Fourier transform infrared spectroscopy (FTIR) analysis demonstrated that there existed molecular interaction between components, which enhanced the mat's thermal stability and mechanic property. It was found that the predominant release mechanism of BSA from fiber mat was erosion, and little change occurred in the secondary structure of encapsulated BSA indicated by FTIR and circular dichroism analysis. The study shows that the obtained fiber mat is a potential delivery system for protein.