Evaluation of citrus somatic hybrids for tolerance to Phytophthora nicotianae and citrus tristeza virus
Evaluation of citrus somatic hybrids for tolerance to Phytophthora nicotianae and citrus tristeza virus
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DOI：
10.1016/j.scienta.2007.10.004
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发表时间：
2008-02-01
影响因子：
4.3
通讯作者：
Cantuarias-Aviles, Tatiana Eugenia
中科院分区：
文献类型：
--
作者：
Alves Mourao Filho, Francisco de Assis;Pio, Rafael;Cantuarias-Aviles, Tatiana Eugenia
体细胞杂交是一种生物技术工具，可用于柑橘育种计划，以产生双亲完全遗传组合的体细胞杂种。本研究的目的是测试柑橘体细胞杂种对烟草疫霉(Phytophthora Nictianae van Breda de Haan，P parasitica Dastur)和柑橘衰退病毒(CTV)引起的树干和根部侵染的反应。所评价的体细胞杂种分别是‘Caipira’甜橙+‘Rangpur’酸橙(C.limonia Osbeck)、‘Caipira’甜橙+‘Cleopatra’柑橘(C.reshni hort)。Ex Tanaka)，‘caipira’甜橙+‘Volkamer’柠檬(C.volkameriana V Ten.)、‘Cleopatra’柑橘+‘Volkamer’柠檬、‘Cleopatra’柑橘+酸橙(C.aurantium L.)、‘Rangpur’酸橙+‘Sunki’柑橘(C.sunki(Hayata)hort.)‘红宝石’甜橙(C.sinensis L.Osbeck)+‘Volkamer’柠檬；‘Rohde Red’甜橙(C.sinensis L.Osbeck)+‘Volkamer’柠檬；‘Valencia’甜橙+Fortunella obovata Hort.前田中。用9个月半硬枝插条获得的体细胞杂种植株，分别在主干接种菌丝和基质中侵染孢子后，与二倍体柑橘砧木品种进行了比较，并对其进行了主干和根部侵染的测定。‘克利奥帕特拉’蜜橘+酸橙、‘朗普’柠檬+‘桑基’蜜橘、‘克利奥帕特拉’蜜橘+‘沃尔卡默’柠檬、‘红宝石’甜橙+‘伏尔卡默’柠檬、‘罗德红’甜橙+‘伏尔卡默’柠檬、‘凯皮拉’甜橙+‘伏尔卡默’柠檬的树干腐烂发生率较低，而体细胞杂种‘克利奥帕特拉’+‘伏尔卡默’柠檬、‘克利奥帕特拉’柑橘+‘伏尔卡默’柠檬、‘凯皮拉’甜橙+‘伏尔卡默’柠檬、‘卡伊拉’甜橙+‘伏尔卡默’柠檬、‘凯皮拉’甜橙+‘伏尔卡默’柠檬耐根腐烂。在CTV检测中，体细胞杂种的植株以及耐受和不耐受的砧木都是用感染CTV的温和菌株或健康的‘Valencia’甜橙芽木萌发的。平均接穗长度的差异表明，‘克利奥帕特拉’柑橘+酸橙和‘瓦伦西亚’甜橙+倒卵金橘对CTV(C)2007 Elsevier B.V.不耐受。
Somatic hybridization is a biotechnology tool that can be used in citrus breeding programs to produce somatic hybrids with the complete genetic combination of both parents. The goal of this work was to test the reaction of citrus somatic hybrids that may be useful as rootstocks to trunk and root infections caused by Phytophthora nicotianae van Breda de Haan (P parasitica Dastur) and to citrus tristeza virus (CTV). The somatic hybrids evaluated were 'Caipira' sweet orange (Citrus sinensis L. Osbeck) + 'Rangpur' lime (C. limonia Osbeck), 'Caipira' sweet orange + 'Cleopatra' mandarin (C. reshni hort. ex Tanaka), 'Caipira' sweet orange + 'Volkamer' lemon (C. volkameriana V Ten. & Pasq.), 'Caipira' sweet orange + rough lemon (C. jambhiri Lush.), 'Cleopatra' mandarin + 'Volkamer' lemon, 'Cleopatra' mandarin + sour orange (C. aurantium L.), 'Rangpur' lime + 'Sunki' mandarin (C. sunki (Hayata) hort. ex Tanaka), 'Ruby Blood' sweet orange (C. sinensis L. Osbeck) + 'Volkamer' lemon, 'Rohde Red' sweet orange (C. sinensis L. Osbeck) + 'Volkamer' lemon, and 'Valencia' sweet orange + Fortunella obovata hort. ex Tanaka. For P. nicotianae trunk and root infection assays, plants of the somatic hybrids, obtained from 9-month semi-hardwood cuttings, were evaluated and compared with diploid citrus rootstock cultivars after mycelia inoculation in the trunk or spore infestation in the substrate, respectively. 'Cleopatra' mandarin + sour orange, 'Rangpur' lime + 'Sunki' mandarin, 'Cleopatra' mandarin + 'Volkamer' lemon, 'Ruby Blood' sweet orange + 'Volkamer' lemon, 'Rohde Red' sweet orange + 'Volkamer' lemon, and 'Caipira' sweet orange + 'Volkamer' lemon had less trunk rot occurrence, whereas the somatic hybrids 'Cleopatra' mandarin + 'Volkamer' lemon, 'Cleopatra' mandarin + sour orange, 'Caipira' sweet orange + 'Volkamer' lemon, and 'Caipira' sweet orange + 'Rangpur' lime were tolerant to root rot. For CTV assays, plants of the somatic hybrids along with tolerant and intolerant rootstocks were budded with a mild strain CTV-infected or healthy 'Valencia' sweet orange budwood. Differences in average scion shoot length indicated that the hybrids 'Cleopatra' mandarin + sour orange and 'Valencia' sweet orange + Fortunella obovata were intolerant to CTV (c) 2007 Elsevier B.V. All rights reserved.