A microsatellite genetic linkage map of human chromosome 13.
A microsatellite genetic linkage map of human chromosome 13.
复制标题
人类13号染色体的微卫星遗传连锁图。
作者：
Petrukhin,KE;Speer,MC;Cayanis,E;Bonaldo,MF;Tantravahi,U;Soares,MB;Fischer,SG;Warburton,D;Gilliam,TC;Ott,J
我们已经鉴定了21个用于绘制13号染色体遗传图谱的多态(CA)微卫星。从流动排序的13号染色体文库中分离到15个标记，4个CA重复序列来自含NotI的粘粒克隆，2个多态标记以前被描述过(J.L.Weber，A.E.Kwitek和P.E.May，1990，Nucleic Res.18：4638；L.Warnich，I.Groenwald，L.Laubscher，和A.E.Retief，1991，AmJ.嗯。吉内。49(补充)：372(摘要))。通过扩增含有13号染色体不同缺失的5个体细胞杂交种的DNA，对所有标记进行了区域定位。遗传标记分布在11个可分辨的染色体亚区中的6个亚区。利用人类多形态研究中心(CEPH)提供的9个家族的数据，绘制了这21个标记中的12个标记的框架图。12个标记中的6个形成三对，每对中的两个成员紧密相连，从而可以区分九个标记系统。这12个标记的平均杂合度为0.75。性别平均图谱的总长度为65.4厘米(科桑比)，标记系统之间的平均距离为8.2厘米(8个间隔)。其余七个标记被临时放置到框架地图中。
We have characterized 21 polymorphixc (CA)nmicrosatellites for the development of a genetic map of chromosome 13. Fifteen markers were isolated from a flow-sorted chromosome 13 library, four CA repeats were derived fromNotI-containing cosmid clones, and two polymorphic markers were described previously (J. L. Weber, A. E. Kwitek, and P. E. May, 1990,Nucleic Acids Res.18: 4638; L. Warnich, I. Groenwald, L. Laubscher, and A. E. Retief, 1991,Am. J. Hum. Genet. 49(Suppl.): 372 (Abstract)). Regional localization for all of the markers was performed by amplification of DNA from five somatic cell hybrids containing different deletions of chromosome 13. Genetic markers were shown to be distributed throughout 6 of the 11 resolvable chromosomal subregions. Using data from nine families provided by the Centre d'Etude du Polymorphisme Humain (CEPH), a framework map of 12 of these 21 markers was developed. Six of the 12 markers form three pairs, with each two members of a pair being tightly linked, such that nine systems of markers can be distinguished. The average heterozygosity of these 12 markers is 0.75. The total length of the sex-averaged map is 65.4 cM (Kosambi), with an average distance of 8.2 cM between systems of markers (eight intervals). Seven remaining markers were placed provisionally into the framework map.