Mice cloned from olfactory sensory neurons
Mice cloned from olfactory sensory neurons
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DOI：
10.1038/nature02375
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发表时间：
2004-03-04
期刊：
影响因子：
64.8
通讯作者：
Jaenisch, R
中科院分区：
文献类型：
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作者：
Eggan, E;Baldwin, K;Jaenisch, R
核移植克隆已在包括小鼠在内的多种哺乳动物中成功进行。迄今为止，小鼠尚未从有丝分裂后细胞（例如神经元）中克隆出来。在这里，我们通过将有丝分裂后的嗅觉感觉神经元的细胞核转移到卵母细胞中，产生了可育的小鼠克隆。这些结果表明，有丝分裂后、终末分化神经元的基因组可以重新进入细胞周期，并在核移植后被重编程为全能状态。此外，克隆小鼠中气味受体基因的表达模式和气味受体基因的组织与野生型动物没有区别，这表明嗅觉神经元DNA的不可逆变化并不伴随着受体基因的选择。
Cloning by nuclear transplantation has been successfully carried out in various mammals, including mice. Until now mice have not been cloned from post-mitotic cells such as neurons. Here, we have generated fertile mouse clones derived by transferring the nuclei of post-mitotic, olfactory sensory neurons into oocytes. These results indicate that the genome of a post-mitotic, terminally differentiated neuron can re-enter the cell cycle and be reprogrammed to a state of totipotency after nuclear transfer. Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice.