Clinical significance and role of LKB1 in gastric cancer
Clinical significance and role of LKB1 in gastric cancer
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LKB1在胃癌中的临床意义和作用
DOI：
10.3892/mmr.2015.4508
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发表时间：
2016-01-01
影响因子：
3.4
通讯作者：
Huang, Deqiang
中科院分区：
文献类型：
--
作者：
Jiang, Shanshan;Chen, Rensheng;Huang, Deqiang
肝激酶B1（LKB 1）是一种肿瘤抑制基因，LKB 1的表达缺失与人类肿瘤的发生和发展有关。本研究旨在探讨LKB 1与胃癌的关系。本研究采用慢病毒转染、逆转录-定量聚合酶链反应（RT-PCR）和流式细胞术检测了63例胃癌患者和胃癌细胞株SGC-7901的表达。通过免疫组化检测LKB 1的表达，发现LKB 1在胃癌组织中的表达降低，而LKB 1表达的恢复降低了肿瘤细胞的存活率、迁移率和CD 44的表达，诱导细胞周期停滞在G2期，并增加了胃癌细胞对抗癌药物的敏感性。LKB 1蛋白是胃癌的抑癌基因，有望成为胃癌基因治疗的新靶点。
Liver kinase B1 (LKB1) functions as a tumor suppressor gene, and loss in the expression of LKB1 contributes to human carcinogenesis and tumor progression. The present study investigated the association between LKB1 and gastric cancer. SGC-7901 gastric cancer cell lines and 63 patients with gastric cancer were examined in the present study, and lentivirus transfection, reverse transription-quantitative polymerase chain reaction and flow cytometric analyses were performed. By examining the expression of LKB1 using immunohistochemical analyses, the present study found that the expression of LKB1 was reduced in the gastric cancer tissues, and restoration of the expression of LKB1 reduced tumor cell viability, migration rate and the expression of CD44, induced cell cycle arrest at the G2 phase of the cell cycle, and increased the sensitivity of the gastric cancer cells to anticancer drugs. LKB1 protein is a tumor-suppressor in gastric cancer and may be potentially be developed as a novel gene therapy target in the treatment of gastric cancer.