Battle for control of anthocyanin biosynthesis in two <i>Brassicaceae</i> species infected with turnip mosaic virus
Battle for control of anthocyanin biosynthesis in two <i>Brassicaceae</i> species infected with turnip mosaic virus
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感染萝卜花叶病毒的两种十字花科植物花青素生物合成的控制之战
DOI：
10.1093/jxb/erac502
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发表时间：
2022
影响因子：
6.9
通讯作者：
Masuta Chikara
中科院分区：
文献类型：
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作者：
Inukai Tsuyoshi;Kim Hangil;Matsunaga Wataru;Masuta Chikara
研究发现，芜菁花叶病毒(TuMV)能显著抑制油菜叶片中花青素的积累，而在同一植株上富含花青素的叶片很少被侵染。为了阐明AA是否是对TuMV的防御，在这项研究中，我们检查了自发性AA的组织水平模式与表达黄色荧光蛋白的TuMV株的细胞定位的关系。我们发现AA显著阻断了TuMV的感染，表明AA对TuMV具有化学屏障作用。接下来，我们分析了受TuMV感染的拟南芥叶片中花青素生物合成相关基因表达的变化。在拟南芥中，TuMV还抑制强光诱导的AA，这种抑制主要是通过抑制花青素后期生物合成基因(LBGS)的表达来实现的。LBGS的大多数阳性转录因子也下调，而负调控因子SPL15则高度上调。黄瓜花叶病毒(CMV)对拟南芥AA也有一定程度的抑制作用，但抑制方式不同。由于富含花色苷的拟南芥叶片似乎对TuMV有抗性，但对CMV没有抗性，我们的结果表明我们观察到的花色苷相关的抗性是TuMV所特有的。
It has previously been found that turnip mosaic virus (TuMV) greatly suppresses anthocyanin accumulation (AA) inBrassica rapaleaves, and that such leaves become infected whilst anthocyanin-enriched leaves on the same plants are rarely infected. To clarify whether AA is a defense against TuMV, in this study we examined tissue-level patterns of spontaneous AA in relation to the cellular localization of a TuMV strain that expresses a yellow fluorescent protein. We found that TuMV infection was significantly blocked by AA, suggesting that it functions as a chemical barrier against TuMV. We next analysed changes in expression of genes related to anthocyanin biosynthesis in TuMV-infected leaves of Arabidopsis. TuMV also suppressed AA that is induced by high light in Arabidopsis, and this this suppression was mainly due to inhibited expression of anthocyanin late-biosynthesis genes (LBGs). Most positive transcription factors of LBGs were also down-regulated, while the negative regulator SPL15 was highly up-regulated. Cucumber mosaic virus (CMV) also moderately suppressed AA in Arabidopsis, but in a different manner. Since it appeared that anthocyanin-enriched leaves of Arabidopsis were resistant to TuMV but not CMV, our results suggested that the anthocyanin-associated resistance that we observed was specific to TuMV.