Arabidopsis CNGC Family Members Contribute to Heavy Metal Ion Uptake in Plants
Arabidopsis CNGC Family Members Contribute to Heavy Metal Ion Uptake in Plants
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拟南芥环核苷酸门控离子通道（CNGC）家族成员参与植物对重金属离子的吸收
DOI：
10.3390/ijms20020413
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发表时间：
2019-01-02
影响因子：
5.6
通讯作者：
Shin, Ryoung
中科院分区：
文献类型：
--
作者：
Moon, Ju Yeon;Belloeil, Celestine;Shin, Ryoung
重金属离子，包括必需离子的毒性浓度，对多种代谢和细胞过程产生负面影响。重金属离子以非选择性的方式进入细胞；然而，很少有研究对重金属离子输运的调控进行研究。植物环核苷酸门控通道（CNGCs）是一种Ca2+可渗透通道，已被认为参与必需和有毒阳离子的摄取。为了确定重金属离子转运的候选基因，研究了一系列拟南芥CNGC突变体对Pb2+和Cd2+离子的响应。研究的重点是根系生长和植物体内重金属浓度的分析。结果表明，AtCNGC1、AtCNGC10、AtCNGC13和AtCNGC19参与拟南芥Pb2+毒性作用，AtCNGC11、AtCNGC13、AtCNGC16和AtCNGC20参与Cd2+毒性作用。离子含量分析证实，AtCNGC1和AtCNGC13突变导致Pb2+积累减少，而AtCNGC11、AtCNGC15和AtCNGC19突变导致Pb2+和Cd2+积累减少。这些发现为AtCNGCs在植物对Pb2+或Cd2+离子的吸收和转运中所起的作用提供了功能证据。
Heavy metal ions, including toxic concentrations of essential ions, negatively affect diverse metabolic and cellular processes. Heavy metal ions are known to enter cells in a non-selective manner; however, few studies have examined the regulation of heavy metal ion transport. Plant cyclic nucleotide-gated channels (CNGCs), a type of Ca2+-permeable-channel, have been suggested to be involved in the uptake of both essential and toxic cations. To determine the candidates responsible for heavy metal ion transport, a series of Arabidopsis CNGC mutants were examined for their response to Pb2+ and Cd2+ ions. The primary focus was on root growth and the analysis of the concentration of heavy metals in plants. Results, based on the analysis of primary root length, indicated that AtCNGC1, AtCNGC10, AtCNGC13 and AtCNGC19 play roles in Pb2+ toxicity, while AtCNGC11, AtCNGC13, AtCNGC16 and AtCNGC20 function in Cd2+ toxicity in Arabidopsis. Ion content analysis verified that the mutations of AtCNGC1 and AtCNGC13 resulted in reduced Pb2+ accumulation, while the mutations of AtCNGC11, AtCNGC15 and AtCNGC19 resulted in less Pb2+ and Cd2+ accumulation in plants. These findings provide functional evidence which support the roles of these AtCNGCs in the uptake and transport of Pb2+ or Cd2+ ion in plants.