Cloning of cDNA for rat pro alpha1(V) collagen mRNA. Expression patterns of type I, type III and type V collagen genes in experimental granulation tissue.
Cloning of cDNA for rat pro alpha1(V) collagen mRNA. Expression patterns of type I, type III and type V collagen genes in experimental granulation tissue.
复制标题
大鼠 pro alpha1(V) 胶原蛋白 mRNA 的 cDNA 克隆。
作者：
K. Inkinen;H. Turakainen;H. Wolff;J. Ahonen
以人和金黄地鼠COL5A1基因为基础，采用聚合酶链式反应(PCR)技术，构建了大鼠前α1(V)胶原基因的cDNA克隆。克隆pRCVA1全长560个核苷酸，编码V型前胶原羧基前肽。与I型胶原序列的同源性为%，与II型胶原的同源性为65%，与III型胶原的同源性为61%。采用大鼠皮下植入粘胶纤维海绵诱导肉芽组织形成的方法，观察V型胶原在创面愈合模型中的时空表达。分别于第5、8、14、21、30、59、84天取材进行肉芽组织分析。用针对前α1(I)、前α1(III)和前α1(V)胶原基因的特异性cDNA探针进行缝隙杂交、Northern杂交和原位杂交。创面愈合初期I型胶原基因表达上调，III型胶原基因表达稳定，14d以后I型和III型胶原基因表达水平基本一致。V型胶原基因在研究的各个时间点均有表达，但表达水平明显低于I型和III型胶原。原位杂交显示，V型胶原在两种不同类型的细胞中均有表达。综上所述，V型胶原在创面愈合模型中至少从第5天开始表达，并由成纤维细胞样细胞和圆形细胞合成。
A cDNA clone for rat pro alpha1(V) collagen mRNA was constructed using PCR amplification, with primers based on human and hamster COL5A1 gene sequences. The clone pRCVA1 is 560 nucleotides long and it encodes for the carboxy propeptide of type V procollagen. Homology shared with type I collagen sequence was 64%, with type II collagen 65% and with type III collagen 61%. To evaluate the spatial and temporal expression of type V collagen mRNA in wound healing model, subcutaneously implanted viscose cellulose sponges in rats were used to induce granulation tissue formation. Analyses on granulation tissue were carried out on days 5, 8, 14, 21, 30, 59 and 84. Specific cDNA probes to pro alpha1(I), pro alpha1(III) and pro alpha1(V) collagen mRNA were used in slot blot, Northern and in situ hybridization. Type I collagen gene expression was upregulated at the initial stage of wound healing, type III collagen gene expression was constant and from the day 14 onwards type I and III collagen gene expressions were at the same level. Type V collagen gene expression was seen at every time point studied but at a considerably lower level than type I and III collagens. In situ hybridization showed that type V collagen was expressed in two different cell types. In conclusion, type V collagen was expressed in the wound healing model from at least day 5 onwards and it was synthesized by fibroblast-like and rounded cells.
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V 型胶原对中性蛋白酶的敏感性：证据表明主要分子种类是凝血酶敏感的杂聚物，[α 1(V)]2 α 2(V)。
影响因子：
2.9
作者：
Sage,H;Pritzl,P;Bornstein,P
通讯作者：
Bornstein,P
酸性β-半乳糖苷酶基因双氨基酸取代多态性对两种近交系小鼠的影响。
DOI：
--
发表时间：
1994
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hara,Y;Nishimoto,J;Suzuki,K
通讯作者：
Suzuki,K
慢性炎症对人体结缔组织中胶原蛋白类型组成的影响。
DOI：
10.1016/s0174-173x(83)80014-4
发表时间：
1983
期刊：
Collagen and related research
影响因子：
--
作者：
Narayanan,AS;Engel,LD;Page,RC
通讯作者：
Page,RC
pro-alpha 1(V) 胶原蛋白链。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Greenspan,DS;Cheng,W;Hoffman,GG
通讯作者：
Hoffman,GG
影响因子：
2.9
作者：
GENOVESE, C;ROWE, D;KREAM, B
通讯作者：
KREAM, B