Granulocyte-Colony Stimulating Factor and Lipopolysaccharide Regulate the Expression of Interleukin 8 Receptors on Polymorphonuclear Leukocytes (*)
Granulocyte-Colony Stimulating Factor and Lipopolysaccharide Regulate the Expression of Interleukin 8 Receptors on Polymorphonuclear Leukocytes (*)
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粒细胞集落刺激因子和脂多糖调节多形核白细胞上白细胞介素8受体的表达(*)
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
D. Kelvin
中科院分区：
文献类型：
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作者：
A. Lloyd;A. Biragyn;J. Johnston;D. Taub;Luoling Xu;D. Michiel;H. Sprenger;J. Oppenheim;D. Kelvin
白细胞介素8 （IL-8）是人类多形核白细胞（PMN）的一种有效的化学引诱剂和激活因子，因此在急性炎症的发病机制中起着关键作用。两个独特但同源的IL-8受体（IL-8RA和-B）已经被克隆出来，它们都能高亲和力地结合IL-8配体。细胞因子或脂多糖（LPS）刺激的PMN表现出IL-8R mRNA和I-IL-8结合的变化。粒细胞集落刺激因子（G-CSF）处理PMN可增强IL-8R mRNA表达，LPS可抑制IL-8R mRNA表达。同样，G-CSF增加了与PMN结合的I-IL-8配体，LPS降低了与PMN的结合。G-CSF对IL-8R表达的刺激作用是转录的，因为它被放线菌素D抑制，并且在核运行分析中很明显。相反，LPS通过转录和转录后机制下调IL-8R。IL-8R表达的改变与il -8诱导PMN趋化反应的类似变化有关。综上所述，两种类型的IL-8受体在细胞分布上存在差异，并受细胞因子和LPS的调节。内源性和外源性免疫调节剂对IL-8R表达的调节可能对炎症中PMN效应物的体内控制很重要。
Interleukin 8 (IL-8) is a potent chemoattractant and activating factor for human polymorphonuclear leukocytes (PMN) and hence plays a critical role in the pathogenesis of acute inflammation. Two unique but homologous receptors for IL-8 have been cloned (IL-8RA and -B), each of which binds the IL-8 ligand with high affinity. PMN stimulated by cytokines or lipopolysaccharide (LPS) exhibit changes in IL-8R mRNA and I-IL-8 binding. Granulocyte-colony stimulating factor (G-CSF) treatment of PMN enhances, and LPS inhibits, IL-8R mRNA expression. Similarly, I-IL-8 ligand binding to PMN is increased by G-CSF and decreased by LPS treatment. The stimulatory effect of G-CSF on IL-8R expression is transcriptional as it is inhibited by actinomycin D and is evident in nuclear run-on analyses. In contrast, LPS down-regulates IL-8R by both transcriptional and post-transcriptional mechanisms. The alterations in IL-8R expression are associated with similar changes in the IL-8-induced chemotactic responses of PMN. In conclusion, the two types of IL-8 receptor differ in their cellular distribution and are regulated in response to cytokines and LPS. Regulation of IL-8R expression by endogenous and exogenous immunomodulators may be important in the in vivo control of PMN effector functions in inflammation.