Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity.
Fluorescence Polarization (FP) Assay for Measuring Staphylococcus aureus Membrane Fluidity.
复制标题
用于测量金黄色葡萄球菌膜流动性的荧光偏振 (FP) 测定。
DOI：
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发表时间：
2021
影响因子：
--
通讯作者：
B. Wilkinson
中科院分区：
文献类型：
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作者：
K. Tiwari;S. Sen;C. Gatto;B. Wilkinson
荧光偏振是一种使用插入脂肪酸双层的疏水荧光染料测定膜流动性的方法。荧光分光光度计用于将UV光作为垂直激发光束，该垂直激发光束穿过染料标记的膜，在染料标记的膜处染料发荧光。然后收集和分析与激发光垂直和水平的光束。膜的结构特性主要是由于脂肪酸在脂质双层中的堆积决定了膜的生物物理参数。金黄色葡萄球菌在膜中含有直链（SCFAs）和支链（BCFAs）脂肪酸，并改变膜流化BCFAs和稳定SCFAs的比例作为对各种应力的响应。本文介绍了一种测定S.使用二苯基己三烯（用于该目的的最常用的荧光染料之一）的金黄色葡萄球菌。
Fluorescence polarization is a method to determine membrane fluidity using a hydrophobic fluorescent dye that intercalates into the fatty acid bilayer. A spectrofluorometer is used to polarize UV light as a vertical excitation beam which passes through the dye-labeled membrane where the dye fluoresces. The beams perpendicular and horizontal to the excitation light are then collected and analyzed. Membrane structural properties are largely due to the packing of the fatty acids in the lipid bilayer that determines the membrane biophysical parameters. Staphylococcus aureus contains straight-chain (SCFAs) and branched-chain (BCFAs) fatty acids in the membrane and alters the proportion of membrane fluidizing BCFAs and stabilizing SCFAs as a response to a variety of stresses. Herein, we describe a method for determination of membrane fluidity in S. aureus using diphenylhexatriene, one of the most used fluorescent dyes for this purpose.
影响因子：
3.4
作者：
Marc Adler;Thomas R. TRIrrON
通讯作者：
Marc Adler;Thomas R. TRIrrON