[Role of ROK in contraction of rat femoral arteries after 14 d tail suspension].
[Role of ROK in contraction of rat femoral arteries after 14 d tail suspension].
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发表时间：
2004-10
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通讯作者：
Zhi-li Li;Ming Yuan;Shi-zhong Jiang;De-sheng Wang;Hui-juan Wang
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作者：
Zhi-li Li;Ming Yuan;Shi-zhong Jiang;De-sheng Wang;Hui-juan Wang
目的探讨Rho相关激酶(ROK)在尾部悬吊14d大鼠股动脉收缩中的作用。方法雄性Wistar大鼠随机分为对照组(CON)和14d-30度尾部悬吊组(TS14d)。分析TS14d和CON大鼠股动脉环灌流后对氯化钾、苯肾上腺素(PE)和U-46619在Y-27632存在下的收缩反应。结果TS14d大鼠动脉环对氯化钾和PE的收缩反应减弱，而对U-46619的收缩反应不明显。在对氯化钾的反应中，Y-27632对TS14d大鼠的组织具有浓度依赖性的松弛作用和明显的收缩作用。Y27632在剂量从10(-6)m ol/L增加到10(-5)m o l/L时，几乎完全消除KCl1诱发的收缩的紧张性成分，对PE和U-46619诱导的收缩也有抑制作用，使U-46619的收缩幅度明显降低，但对KCl2和PE诱导的收缩作用无明显影响。结论TS14d可增强大鼠股动脉的ROK活性并起到代偿作用。
OBJECTIVE To determine the role of Rho associated kinase (ROK) in contraction of rat femoral arteries after 14 d tail suspension. METHOD Male Wistar rats were randomly divided into control group (CON) and 14 d -30 degrees tail suspension group (TS14d). Analysis was performed on the contractile responses of perfused femoral arterial rings from both TS14d and CON rats to KCl, phenylephrine (PE), and U-46619 in the presence of Y-27632. RESULT Arterial rings from TS14d rats displayed a reduced contractile response to KCl and PE but not significantly to U-46619. In the response to KCl, Y-27632 caused a concentration-dependent relaxation and significantly larger decrease of contraction in tissues from TS14d rats. Y27632 nearly abolished the tonic component of KCl-induced contraction when its dose was increased from 10(-6)mol/L to 10(-5) mol/L. It had also an inhibitive effect on the PE and U-46619-induced contraction and caused significantly larger decrease in U-46619 but not in KCl or PE induced contraction in tissues from TS14d rats. CONCLUSION ROK activity may be enhanced and play a compensational role in rat femoral arteries after TS14d.