Metabolic changes in the anterior and posterior cingulate cortices of the normal aging brain: proton magnetic resonance spectroscopy study at 3 T
Metabolic changes in the anterior and posterior cingulate cortices of the normal aging brain: proton magnetic resonance spectroscopy study at 3 T
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DOI：
10.1007/s11357-013-9545-8
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发表时间：
2014-02-01
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影响因子：
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通讯作者：
Chu, Leung-Wing
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文献类型：
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作者：
Chiu, Pui-Wai;Mak, Henry Ka-Fung;Chu, Leung-Wing
磁共振波谱（MRS）可以在分子水平上探索衰老。在这项研究中，我们研究了区域浓度的代谢物（如胆碱，肌酸，肌醇，N-乙酰天冬氨酸）和正常老化之间的关系，在30个认知正常的受试者（15名女性和15名男性，年龄范围22-82，平均= 49.9 +/- 18.3岁）使用定量质子磁共振波谱。所有MR扫描均使用3 T扫描仪进行。点分辨光谱被用作感兴趣区域的体积选择方法和水抑制的激发方法。采用单体素光谱，回波时间为39 ms，重复时间为2,000 ms。单个体素被放置在边缘区域，即，前扣带皮层（ACC）、后扣带皮层（PCC）以及左和右扣带皮层。在计算绝对代谢物浓度时，采用脑脊液标准化以及T1和T2校正因子。还采集了标准化T1 W 3D体积快速场回波和轴向T2加权快速自旋回波图像。我们的结果表明，胆碱呈显著正相关（r = 0.545，p = 0.002），肌酸（r = 0.571，p = 0.001）和N-乙酰基-天冬氨酸（r = 0.674，p < 0.001）在ACC中;胆碱（r = 0.614，p < 0.001），肌酸（r = 0.670，p < 0.001），N-乙酰-天冬氨酸（r = 0.528，p = 0.003），左侧海马NAA（r = 0.409，p = 0.025）。代谢物浓度没有显着的性别效应被发现。在老化，胆碱和肌酸的增加可能表明神经胶质细胞增殖，N-乙酰天冬氨酸的增加可能表明神经元肥大。这些发现突出了ACC和PCC随年龄的代谢变化，这可能是对能量需求增加和脑血流量降低的补偿。
Magnetic resonance spectroscopy (MRS) can explore aging at a molecular level. In this study, we investigated the relationships between regional concentrations of metabolites (such as choline, creatine, myo-inositol, and N-acetyl-aspartate) and normal aging in 30 cognitively normal subjects (15 women and 15 men, age range 22-82, mean = 49.9 +/- 18.3 years) using quantitative proton magnetic resonance spectroscopy. All MR scans were performed using a 3 T scanner. Point resolved spectroscopy was used as the volume selection method for the region-of-interest and the excitation method for water suppression. Single voxel spectroscopy with short echo time of 39 ms and repetition time of 2,000 ms was employed. Single voxels were placed in the limbic regions, i.e., anterior cingulate cortex (ACC), posterior cingulate cortex (PCC), and left and right hippocampi. Cerebrospinal fluid normalization and T1 and T2 correction factors were implemented in the calculation of absolute metabolite concentrations. A standardized T1W 3D volumetric fast field echo and axial T2-weighted fast spin-echo images were also acquired. Our results showed significant positive correlation of choline (r = 0.545, p = 0.002), creatine (r = 0.571, p = 0.001), and N-acetyl-aspartate (r = 0.674, p < 0.001) in the ACC; choline (r = 0.614, p < 0.001), creatine (r = 0.670, p < 0.001), and N-acetyl-aspartate (r = 0.528, p = 0.003) in the PCC; and NAA (r = 0.409, p = 0.025) in the left hippocampus, with age. No significant gender effect on metabolite concentrations was found. In aging, increases in choline and creatine might suggest glial proliferation, and an increase in N-acetyl-aspartate might indicate neuronal hypertrophy. Such findings highlight the metabolic changes of ACC and PCC with age, which could be compensatory to an increased energy demand coupled with a lower cerebral blood flow.