INTRACELLULAR FREE CALCIUM LEVELS ARE REDUCED IN MITOTIC PT-K2 EPITHELIAL-CELLS
INTRACELLULAR FREE CALCIUM LEVELS ARE REDUCED IN MITOTIC PT-K2 EPITHELIAL-CELLS
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DOI：
10.1073/pnas.82.3.800
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
SHELANSKI, ML
中科院分区：
文献类型：
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作者：
KEITH, CH;MAXFIELD, FR;SHELANSKI, ML
用荧光比值法在显微镜下研究了单个负载quin-2的有丝分裂细胞内游离钙水平。Pt K2上皮细胞中的细胞内游离Ca浓度下降约在有丝分裂过程中占50%。间期细胞Ca ~（2+）水平为53 . 7nM。在分裂前期，胞质游离Ca开始下降，达到28 ± 100。前中期3 nM。钙水平保持较低，直到核膜在末期后期重新形成，当它们再次增加到间期水平。有丝分裂中游离钙的总体减少表明，有丝分裂细胞具有钙离子的一般螯合和可能的局部释放的机制。
A fluorescene ratio method was used to study the intracellular free Ca levels in individual quin-2-loaded mitotic cells under the microscope. Intracellular free Ca concentrations in Pt K2 epithelial cells drop by .apprxeq. 50% as they pass through mitosis. Ca levels in interphase cells were 53 .+-. 7 nM. During prophase, free cytoplamic Ca begins to decrease, reaching 28 .+-. 3 nM in prometaphase. Ca levels remain low until the nuclear envelope is re-formed in late telophase, when they increase again to interphase levels. This decrease in overall free calcium in mitosis suggests that the mitotic cell has mechanisms for the general sequestration, and perhaps local release, of Ca ions.